Phylogenetic diversity and characterization of 2-haloacid degrading bacteria from the marine sponge Hymeniacidon perlevis

Jianyu Huang, Yanjuan Xin, Xupeng Cao, Wei Zhang

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    11 Citations (Scopus)

    Abstract

    A total of 139 2-haloacid degrading bacteria strains were isolated from the marine sponge Hymeniacidon perlevis using a modified enrichment medium and a pH indicator method. After screening on indicator agar and 2-chloropropionic acid (2-CPA) liquid medium, 11 isolates with high degrading activities were characterized and initially identified. Seven of the 11 isolates were able to degrade 2-CPA at 8% salt, and four isolates (DEH 66, DEH 99, DEH125 and DEH138) degraded 2-CPA at 15% salt. Eight of the 11 isolates utilized all four types of organohalogen compounds used in this study. The DEH99 and DEH138 isolates exhibited the best enantioselectivity towards (S)-2-chloropropionic acid (S-CPA) and (R)-2-chloropropionic acid (R-CPA), respectively. The dehalogenase activities of DEH84 against racemic CPA, DEH99 against S-CPA, DEH138 against R-CPA and DEH130 against racemic CPA were 0. 16U/mg, 0. 06U/mg, 0. 12U/mg and 0. 19U/mg, respectively. Based on 16S rRNA sequence analysis, the 11 isolates were clustered into the Rhodobacteraceae family of α-proteobacteria and the Pseudomonadaceae family of γ-proteobacteria. To our knowledge, this is the first report detailing the isolation of organisms of Pseudomonas stuzeri sp. and the Rhodobacteraceae family with 2-haloacid dehalogenase activity from marine sponges.

    Original languageEnglish
    Pages (from-to)1787-1794
    Number of pages8
    JournalWorld Journal of Microbiology and Biotechnology
    Volume27
    Issue number8
    DOIs
    Publication statusPublished - Aug 2011

    Keywords

    • 2-haloacid dehalogenase
    • Bacteria
    • Enantioselectivity
    • Marine sponge
    • Salt tolerance

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