PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20

Ian Nicholson, C Mavrangelos, Daniel Bird, Suzanne Bresatz-Atkins, Nicola Eastaff-Leung, Randall Grose, Batjargal Gundsambuu, Danika Hill, Debbrah Millard, Timothy Sadlon, sarah to, Heddy Zola, Simon Barry, Doreen Krumbiegel

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    14 Citations (Scopus)

    Abstract

    The peptidase inhibitor PI16 was shown previously by microarray analysis to be over-expressed by CD4-positive/CD25-positive Treg compared with CD4-positive/CD25-negative Th cells. Using a monoclonal antibody to the human PI16 protein, we found that PI16-positive Treg have a memory (CD45RO-positive) phenotype and express higher levels of FOXP3 than PI16-negative Treg. PI16-positive Treg are functional in suppressor assays . in vitro with potency similar to PI16-negative Treg. Further phenotyping of the PI16-positive Treg revealed that the chemokine receptors CCR4 and CCR6 are expressed by more of the PI16-positive/CD45RO-positive Treg compared with PI16-negative/CD45RO-positive Treg or Th cells. PI16-positive Treg showed enhanced . in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation. We conclude that PI16 identifies a novel distinct subset of functional memory Treg which can migrate to sites of inflammation and regulate the pro-inflammatory response at those sites.

    Original languageEnglish
    Pages (from-to)12-18
    Number of pages7
    JournalCellular Immunology
    Volume275
    Issue number1-2
    DOIs
    Publication statusPublished - 2012

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  • Cite this

    Nicholson, I., Mavrangelos, C., Bird, D., Bresatz-Atkins, S., Eastaff-Leung, N., Grose, R., Gundsambuu, B., Hill, D., Millard, D., Sadlon, T., to, S., Zola, H., Barry, S., & Krumbiegel, D. (2012). PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20. Cellular Immunology, 275(1-2), 12-18. https://doi.org/10.1016/j.cellimm.2012.04.002