PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20

Ian Nicholson; C Mavrangelos; Daniel Bird; Suzanne Bresatz-Atkins; Nicola Eastaff-Leung; Randall Grose; Batjargal Gundsambuu; Danika Hill; Debbrah Millard; Timothy Sadlon; sarah to; Heddy Zola; Simon Barry; Doreen Krumbiegel

doi:10.1016/j.cellimm.2012.04.002

PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20

Ian Nicholson, C Mavrangelos, Daniel Bird, Suzanne Bresatz-Atkins, Nicola Eastaff-Leung, Randall Grose, Batjargal Gundsambuu, Danika Hill, Debbrah Millard, Timothy Sadlon, sarah to, Heddy Zola, Simon Barry, Doreen Krumbiegel

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18 Citations (Scopus)

Abstract

The peptidase inhibitor PI16 was shown previously by microarray analysis to be over-expressed by CD4-positive/CD25-positive Treg compared with CD4-positive/CD25-negative Th cells. Using a monoclonal antibody to the human PI16 protein, we found that PI16-positive Treg have a memory (CD45RO-positive) phenotype and express higher levels of FOXP3 than PI16-negative Treg. PI16-positive Treg are functional in suppressor assays . in vitro with potency similar to PI16-negative Treg. Further phenotyping of the PI16-positive Treg revealed that the chemokine receptors CCR4 and CCR6 are expressed by more of the PI16-positive/CD45RO-positive Treg compared with PI16-negative/CD45RO-positive Treg or Th cells. PI16-positive Treg showed enhanced . in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation. We conclude that PI16 identifies a novel distinct subset of functional memory Treg which can migrate to sites of inflammation and regulate the pro-inflammatory response at those sites.

Original language	English
Pages (from-to)	12-18
Number of pages	7
Journal	Cellular Immunology
Volume	275
Issue number	1-2
DOIs	https://doi.org/10.1016/j.cellimm.2012.04.002
Publication status	Published - Jan 2012

Keywords

Lymphocyte migration
Memory Treg
Peptidase inhibitor 16
Regulatory T cells

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10.1016/j.cellimm.2012.04.002

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Nicholson, I., Mavrangelos, C., Bird, D., Bresatz-Atkins, S., Eastaff-Leung, N., Grose, R., Gundsambuu, B., Hill, D., Millard, D., Sadlon, T., to, S., Zola, H., Barry, S., & Krumbiegel, D. (2012). PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20. Cellular Immunology, 275(1-2), 12-18. https://doi.org/10.1016/j.cellimm.2012.04.002

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title = "PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20",

abstract = "The peptidase inhibitor PI16 was shown previously by microarray analysis to be over-expressed by CD4-positive/CD25-positive Treg compared with CD4-positive/CD25-negative Th cells. Using a monoclonal antibody to the human PI16 protein, we found that PI16-positive Treg have a memory (CD45RO-positive) phenotype and express higher levels of FOXP3 than PI16-negative Treg. PI16-positive Treg are functional in suppressor assays . in vitro with potency similar to PI16-negative Treg. Further phenotyping of the PI16-positive Treg revealed that the chemokine receptors CCR4 and CCR6 are expressed by more of the PI16-positive/CD45RO-positive Treg compared with PI16-negative/CD45RO-positive Treg or Th cells. PI16-positive Treg showed enhanced . in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation. We conclude that PI16 identifies a novel distinct subset of functional memory Treg which can migrate to sites of inflammation and regulate the pro-inflammatory response at those sites.",

keywords = "Lymphocyte migration, Memory Treg, Peptidase inhibitor 16, Regulatory T cells",

author = "Ian Nicholson and C Mavrangelos and Daniel Bird and Suzanne Bresatz-Atkins and Nicola Eastaff-Leung and Randall Grose and Batjargal Gundsambuu and Danika Hill and Debbrah Millard and Timothy Sadlon and sarah to and Heddy Zola and Simon Barry and Doreen Krumbiegel",

year = "2012",

month = jan,

doi = "10.1016/j.cellimm.2012.04.002",

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Nicholson, I, Mavrangelos, C, Bird, D, Bresatz-Atkins, S, Eastaff-Leung, N, Grose, R, Gundsambuu, B, Hill, D, Millard, D, Sadlon, T, to, S, Zola, H, Barry, S & Krumbiegel, D 2012, 'PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20', Cellular Immunology, vol. 275, no. 1-2, pp. 12-18. https://doi.org/10.1016/j.cellimm.2012.04.002

TY - JOUR

T1 - PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20

AU - Nicholson, Ian

AU - Mavrangelos, C

AU - Bird, Daniel

AU - Bresatz-Atkins, Suzanne

AU - Eastaff-Leung, Nicola

AU - Grose, Randall

AU - Gundsambuu, Batjargal

AU - Hill, Danika

AU - Millard, Debbrah

AU - Sadlon, Timothy

AU - to, sarah

AU - Zola, Heddy

AU - Barry, Simon

AU - Krumbiegel, Doreen

PY - 2012/1

Y1 - 2012/1

N2 - The peptidase inhibitor PI16 was shown previously by microarray analysis to be over-expressed by CD4-positive/CD25-positive Treg compared with CD4-positive/CD25-negative Th cells. Using a monoclonal antibody to the human PI16 protein, we found that PI16-positive Treg have a memory (CD45RO-positive) phenotype and express higher levels of FOXP3 than PI16-negative Treg. PI16-positive Treg are functional in suppressor assays . in vitro with potency similar to PI16-negative Treg. Further phenotyping of the PI16-positive Treg revealed that the chemokine receptors CCR4 and CCR6 are expressed by more of the PI16-positive/CD45RO-positive Treg compared with PI16-negative/CD45RO-positive Treg or Th cells. PI16-positive Treg showed enhanced . in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation. We conclude that PI16 identifies a novel distinct subset of functional memory Treg which can migrate to sites of inflammation and regulate the pro-inflammatory response at those sites.

AB - The peptidase inhibitor PI16 was shown previously by microarray analysis to be over-expressed by CD4-positive/CD25-positive Treg compared with CD4-positive/CD25-negative Th cells. Using a monoclonal antibody to the human PI16 protein, we found that PI16-positive Treg have a memory (CD45RO-positive) phenotype and express higher levels of FOXP3 than PI16-negative Treg. PI16-positive Treg are functional in suppressor assays . in vitro with potency similar to PI16-negative Treg. Further phenotyping of the PI16-positive Treg revealed that the chemokine receptors CCR4 and CCR6 are expressed by more of the PI16-positive/CD45RO-positive Treg compared with PI16-negative/CD45RO-positive Treg or Th cells. PI16-positive Treg showed enhanced . in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation. We conclude that PI16 identifies a novel distinct subset of functional memory Treg which can migrate to sites of inflammation and regulate the pro-inflammatory response at those sites.

KW - Lymphocyte migration

KW - Memory Treg

KW - Peptidase inhibitor 16

KW - Regulatory T cells

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DO - 10.1016/j.cellimm.2012.04.002

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SN - 0008-8749

VL - 275

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EP - 18

JO - Cellular Immunology

JF - Cellular Immunology

IS - 1-2

ER -

PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20

Abstract

Keywords

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