Plasma Polymer and PEG-Based Coatings for DNA, Protein and Cell Microarrays

Andrew Hook, Nicolas Voelcker, Helmut Thissen

    Research output: Chapter in Book/Report/Conference proceedingChapter

    1 Citation (Scopus)

    Abstract

    DNA, protein and cell microarrays are increasingly used in a multitude of bioassays. All of these arrays require substrates that are suitable for the immobilisation and display of arrayed probe molecules whilst at the same time resisting non-specific interactions of biomolecules with the substrate in areas between printed spots. To meet these conflicting requirements, three different approaches have been developed, all of which were based on low-fouling, high-density poly(ethylene glycol) (PEG) background coatings. In the first approach, the coating was based on allylamine plasma polymerisation (ALAPP) and the subsequent high-density grafting of PEG, followed by the generation of a surface chemical pattern using laser ablation. In the second approach, a photoreactive polymer was printed on the same ALAPP-PEG background. The third approach was based on ALAPP deposition followed by the formation of a multifunctional layer by spin coating a PEG-based polymer that also displayed epoxy groups. The successful demonstration of DNA, protein and cell microarrays has been achieved on each of these coatings.

    Original languageEnglish
    Title of host publicationCell-Based Microarrays Methods and Protocols
    PublisherHUMANA PRESS
    Pages159-170
    Number of pages12
    ISBN (Print)9781617379697
    DOIs
    Publication statusPublished - 2011

    Publication series

    NameMethods in Molecular Biology
    Volume706
    ISSN (Print)1064-3745

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  • Cite this

    Hook, A., Voelcker, N., & Thissen, H. (2011). Plasma Polymer and PEG-Based Coatings for DNA, Protein and Cell Microarrays. In Cell-Based Microarrays Methods and Protocols (pp. 159-170). (Methods in Molecular Biology; Vol. 706). HUMANA PRESS. https://doi.org/10.1007/978-1-61737-970-3_13