Plasma Polymer and PEG-Based Coatings for DNA, Protein and Cell Microarrays

Andrew Hook, Nicolas Voelcker, Helmut Thissen

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    3 Citations (Scopus)

    Abstract

    DNA, protein and cell microarrays are increasingly used in a multitude of bioassays. All of these arrays require substrates that are suitable for the immobilisation and display of arrayed probe molecules whilst at the same time resisting non-specific interactions of biomolecules with the substrate in areas between printed spots. To meet these conflicting requirements, three different approaches have been developed, all of which were based on low-fouling, high-density poly(ethylene glycol) (PEG) background coatings. In the first approach, the coating was based on allylamine plasma polymerisation (ALAPP) and the subsequent high-density grafting of PEG, followed by the generation of a surface chemical pattern using laser ablation. In the second approach, a photoreactive polymer was printed on the same ALAPP-PEG background. The third approach was based on ALAPP deposition followed by the formation of a multifunctional layer by spin coating a PEG-based polymer that also displayed epoxy groups. The successful demonstration of DNA, protein and cell microarrays has been achieved on each of these coatings.

    Original languageEnglish
    Title of host publicationCell-Based Microarrays Methods and Protocols
    PublisherHUMANA PRESS
    Pages159-170
    Number of pages12
    ISBN (Print)9781617379697
    DOIs
    Publication statusPublished - 2011

    Publication series

    NameMethods in Molecular Biology
    Volume706
    ISSN (Print)1064-3745

    Keywords

    • Contact printing
    • Inkjet printing
    • Laser ablation
    • Microarrays
    • Patterned substrates
    • PEG
    • Plasma polymerisation

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