Plasma quantitation of warfarin and warfarin alcohol by gas chromatography chemical ionization mass spectrometry in patients on warfarin maintenance therapy

A. M. Duffield, P. H. Duffield, D. J. Birkett, M Kennedy, D. N. Wade

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A quantitative method has been developed to measure plasma concentrations of warfarin and warfarin alcohol. The analytical procedure uses deuterated analogues as internal standards, and the technique of selected ion monitoring following gas chromatography methane chemical ionization mass spectrometry of the 4′‐methyl ethers of warfarin and warfarin alcohol. Concentrations of warfarin and warfarin alcohol have been measured in plasma samples from 43 patients maintained on chronic warfarin therapy and compared with the ‘apparent warfarin’ concentration as measured by a fluorometric procedure. The study demonstrated a high degree of correlation between the gas chromatographic mass spectrometric derived sum of the individual concentrations of warfarin and warfarin alcohol, and the ‘apparent warfarin’ concentration determined from a spectrofluorometric assay.

Original languageEnglish
Pages (from-to)208-211
Number of pages4
JournalBiological Mass Spectrometry
Volume6
Issue number5
DOIs
Publication statusPublished - May 1979
Externally publishedYes

Fingerprint Dive into the research topics of 'Plasma quantitation of warfarin and warfarin alcohol by gas chromatography chemical ionization mass spectrometry in patients on warfarin maintenance therapy'. Together they form a unique fingerprint.

  • Cite this