Introduction Diabetes is a major health problem in South Africa. DiabCare Africa found just 47% of diabetes patients had a hemoglobin A1c (HbA1c) test for their management in the previous year. Methods Patients attending an urban diabetes clinic near Johannesburg, run by Project HOPE, accessed HbA1c (and urine albumin:creatinine ratio) point-of-care testing (POCT) as part of a quality-assured international program called ACE (Analytical and Clinical Excellence). Patients who had two or more HbA1c POC tests from 2012 to 2014 were assessed to determine their change in glycaemic control. Results The mean (±SD) HbA1c in this group of diabetes patients (n = 131) fell significantly from 9.7% ± 2.4 (83 mmol/mol) at their first POCT measurement to 8.4% ± 2.4 (68 mmol/mmol/mol) at their most recent POCT measurement (paired t-test p < 0.01). The average time between first and most recent HbA1c test was 15 months. The number of diabetes patients achieving optimal glycaemic control (HbA1c ≤ 6.5–7.5% [48–58 mmol/mol) increased by 125%, while the number with very poor glycaemic control (HbA1c > 10% [86 mmol/mol]) halved. An association was observed between degree of glycaemic control and increasing albuminuria in this cohort. Discussion POCT has promoted change in clinical practice by facilitating greater accessibility to HbA1c testing.