Pollen baiting facilitates the isolation of marine thraustochytrids with potential in omega-3 and biodiesel production

Adarsha Gupta, Serena Wilkens, Jacqui Adcock, Munish Puri, Colin Barrow

    Research output: Contribution to journalArticlepeer-review

    27 Citations (Scopus)

    Abstract

    Marine heterotrophic microbes are capable of accumulating large amounts of lipids, omega-3 fatty acids, carotenoids, and have potential for biodiesel production. Pollen baiting using Pinus radiata pollen grain along with direct plating techniques were used in this study as techniques for the isolation of oil-producing marine thraustochytrid species from Queenscliff, Victoria, Australia. Thirteen isolates were obtained using either direct plating or using pine pollen, with pine pollen acting as a specific substrate for the surface attachment of thraustochytrids. The isolates obtained from the pollen baiting technique showed a wide range of docosahexaenoic acid (DHA) accumulation, from 11 to 41 % of total fatty acid content (TFA). Direct plating isolates showed a moderate range of DHA accumulation, from 19 to 25 % of TFA. Seven isolates were identified on the basis of 18S rRNA sequencing technique as Thraustochytrium species, Schizochytrium species, and Ulkenia species. Although both methods appear to result in the isolation of similar strains, pollen baiting proved to be a simpler method for the isolation of these relatively slow-growing organisms.

    Original languageEnglish
    Pages (from-to)1231-1240
    Number of pages10
    JournalJOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
    Volume40
    Issue number11
    DOIs
    Publication statusPublished - 2013

    Keywords

    • 18S rRNA
    • Biodiesel
    • Direct plating
    • Isolation
    • Pollen grain
    • PUFAs
    • Schizochytrium sp.
    • Thraustochytrium sp.

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