TY - JOUR
T1 - Poly(I:C)-induced tumour cell death leads to DC maturation and Th1 activation
AU - Kovalcsik, Edit
AU - Lowe, Katie
AU - Fischer, Mike
AU - Dalgleish, Angus
AU - Bodman-Smith, Mark D.
PY - 2011/11
Y1 - 2011/11
N2 - Dendritic cells (DCs) have the ability to generate peptide epitopes for MHC class I molecules derived from apoptotic tumour cells for direct recognition by cytotoxic T cells. This function has lead to DCs being used in vaccine strategies. In this study, we investigate the effect of inducing apoptosis in tumour cell lines using IFN-γ and poly(I:C), the subsequent maturation of the endocytosing DC and its ability to direct the resulting T cell response. We show that uptake of poly(I:C)-induced apoptotic tumour cells leads to DC maturation and activation with a Th1 cell polarising capacity. In contrast, these effects are not seen by DCs loaded with γ-irradiated apoptotic tumour cells. We propose that the manner in which tumour cells are induced to die can have a profound effect on the endocytosing DC and the resulting T cell response.
AB - Dendritic cells (DCs) have the ability to generate peptide epitopes for MHC class I molecules derived from apoptotic tumour cells for direct recognition by cytotoxic T cells. This function has lead to DCs being used in vaccine strategies. In this study, we investigate the effect of inducing apoptosis in tumour cell lines using IFN-γ and poly(I:C), the subsequent maturation of the endocytosing DC and its ability to direct the resulting T cell response. We show that uptake of poly(I:C)-induced apoptotic tumour cells leads to DC maturation and activation with a Th1 cell polarising capacity. In contrast, these effects are not seen by DCs loaded with γ-irradiated apoptotic tumour cells. We propose that the manner in which tumour cells are induced to die can have a profound effect on the endocytosing DC and the resulting T cell response.
KW - Apoptosis
KW - Dendritic cells
KW - Poly(I:C)
KW - T helper cell type 1
KW - Tumour cells
UR - http://www.scopus.com/inward/record.url?scp=82355185927&partnerID=8YFLogxK
U2 - 10.1007/s00262-011-1058-7
DO - 10.1007/s00262-011-1058-7
M3 - Article
C2 - 21691724
AN - SCOPUS:82355185927
VL - 60
SP - 1609
EP - 1624
JO - Cancer Immunology, Immunotherapy
JF - Cancer Immunology, Immunotherapy
SN - 0340-7004
IS - 11
ER -