Poly(I:C)-induced tumour cell death leads to DC maturation and Th1 activation

Edit Kovalcsik, Katie Lowe, Mike Fischer, Angus Dalgleish, Mark D. Bodman-Smith

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Dendritic cells (DCs) have the ability to generate peptide epitopes for MHC class I molecules derived from apoptotic tumour cells for direct recognition by cytotoxic T cells. This function has lead to DCs being used in vaccine strategies. In this study, we investigate the effect of inducing apoptosis in tumour cell lines using IFN-γ and poly(I:C), the subsequent maturation of the endocytosing DC and its ability to direct the resulting T cell response. We show that uptake of poly(I:C)-induced apoptotic tumour cells leads to DC maturation and activation with a Th1 cell polarising capacity. In contrast, these effects are not seen by DCs loaded with γ-irradiated apoptotic tumour cells. We propose that the manner in which tumour cells are induced to die can have a profound effect on the endocytosing DC and the resulting T cell response.

Original languageEnglish
Pages (from-to)1609-1624
Number of pages16
JournalCancer Immunology, Immunotherapy
Issue number11
Publication statusPublished - Nov 2011
Externally publishedYes


  • Apoptosis
  • Dendritic cells
  • Poly(I:C)
  • T helper cell type 1
  • Tumour cells


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