Abstract
Dendritic cells (DCs) have the ability to generate peptide epitopes for MHC class I molecules derived from apoptotic tumour cells for direct recognition by cytotoxic T cells. This function has lead to DCs being used in vaccine strategies. In this study, we investigate the effect of inducing apoptosis in tumour cell lines using IFN-γ and poly(I:C), the subsequent maturation of the endocytosing DC and its ability to direct the resulting T cell response. We show that uptake of poly(I:C)-induced apoptotic tumour cells leads to DC maturation and activation with a Th1 cell polarising capacity. In contrast, these effects are not seen by DCs loaded with γ-irradiated apoptotic tumour cells. We propose that the manner in which tumour cells are induced to die can have a profound effect on the endocytosing DC and the resulting T cell response.
Original language | English |
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Pages (from-to) | 1609-1624 |
Number of pages | 16 |
Journal | Cancer Immunology, Immunotherapy |
Volume | 60 |
Issue number | 11 |
DOIs | |
Publication status | Published - Nov 2011 |
Externally published | Yes |
Keywords
- Apoptosis
- Dendritic cells
- Poly(I:C)
- T helper cell type 1
- Tumour cells