Polymer-peptide hybrids as a highly immunogenic single-dose nanovaccine

Abdullah Ah Ahmad Fuaad, Zhongfan Jia, Mehfuz Zaman, Jon Hartas, Zyta M. Ziora, I. Chun Lin, Peter M. Moyle, Michael R. Batzloff, Michael F. Good, Michael J. Monteiro, Mariusz Skwarczynski, Istvan Toth

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31 Citations (Scopus)

Abstract

Aim: To explore four-arm star poly(t-butyl)acrylate (PtBA)- peptide and linear PtBA-peptide conjugates as a vaccine-delivery system against Group A Streptococcus. Materials & methods: PtBA nanoparticles bearing J14 peptide epitopes were prepared via alkyne-azide 1,3-dipolar cycloaddition 'click reaction. The conjugated products were self-assembled into small or large nanoparticles. These nanoparticle vaccine candidates were evaluated in vivo and J14-specific antibody titers were assessed. Results & discussion: Mice vaccinated with the nanoparticles were able to produce J14-specific IgG antibodies without the use of an external adjuvant after a single immunization. We have demonstrated for the first time that the immune responses against self-assembled PtBA nanoparticles are stronger for the smaller sized (̃20 nm) nanoparticles compared with the larger (̃500nm) PtBA nanoparticles. Conclusion: PtBA analogs have the potential to be developed as potent carrier systems for single-dose synthetic vaccines.

Original languageEnglish
Pages (from-to)35-43
Number of pages9
JournalNanomedicine
Volume9
Issue number1
Early online date23 Apr 2013
DOIs
Publication statusPublished - 19 Dec 2013
Externally publishedYes

Keywords

  • nanoparticle
  • particle size
  • peptide-based subunit vaccine
  • poly(t-butyl)acrylate
  • self-adjuvanting vaccine
  • single immunization
  • Streptococcus pyogenes
  • vaccine delivery
  • vaccine formulation

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  • Cite this

    Ahmad Fuaad, A. A., Jia, Z., Zaman, M., Hartas, J., Ziora, Z. M., Lin, I. C., Moyle, P. M., Batzloff, M. R., Good, M. F., Monteiro, M. J., Skwarczynski, M., & Toth, I. (2013). Polymer-peptide hybrids as a highly immunogenic single-dose nanovaccine. Nanomedicine, 9(1), 35-43. https://doi.org/10.2217/nnm.13.7