Polymer-peptide hybrids as a highly immunogenic single-dose nanovaccine

Abdullah Ah Ahmad Fuaad; Zhongfan Jia; Mehfuz Zaman; Jon Hartas; Zyta M. Ziora; I. Chun Lin; Peter M. Moyle; Michael R. Batzloff; Michael F. Good; Michael J. Monteiro; Mariusz Skwarczynski; Istvan Toth

doi:10.2217/nnm.13.7

Polymer-peptide hybrids as a highly immunogenic single-dose nanovaccine

Abdullah Ah Ahmad Fuaad, Zhongfan Jia, Mehfuz Zaman, Jon Hartas, Zyta M. Ziora, I. Chun Lin, Peter M. Moyle, Michael R. Batzloff, Michael F. Good, Michael J. Monteiro, Mariusz Skwarczynski, Istvan Toth

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

Aim: To explore four-arm star poly(t-butyl)acrylate (P^tBA)- peptide and linear P^tBA-peptide conjugates as a vaccine-delivery system against Group A Streptococcus. Materials & methods: P^tBA nanoparticles bearing J14 peptide epitopes were prepared via alkyne-azide 1,3-dipolar cycloaddition 'click reaction. The conjugated products were self-assembled into small or large nanoparticles. These nanoparticle vaccine candidates were evaluated in vivo and J14-specific antibody titers were assessed. Results & discussion: Mice vaccinated with the nanoparticles were able to produce J14-specific IgG antibodies without the use of an external adjuvant after a single immunization. We have demonstrated for the first time that the immune responses against self-assembled P^tBA nanoparticles are stronger for the smaller sized (̃20 nm) nanoparticles compared with the larger (̃500nm) P^tBA nanoparticles. Conclusion: PtBA analogs have the potential to be developed as potent carrier systems for single-dose synthetic vaccines.

Original language	English
Pages (from-to)	35-43
Number of pages	9
Journal	Nanomedicine
Volume	9
Issue number	1
Early online date	23 Apr 2013
DOIs	https://doi.org/10.2217/nnm.13.7
Publication status	Published - 19 Dec 2013
Externally published	Yes

Keywords

nanoparticle
particle size
peptide-based subunit vaccine
poly(t-butyl)acrylate
self-adjuvanting vaccine
single immunization
Streptococcus pyogenes
vaccine delivery
vaccine formulation

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title = "Polymer-peptide hybrids as a highly immunogenic single-dose nanovaccine",

abstract = "Aim: To explore four-arm star poly(t-butyl)acrylate (PtBA)- peptide and linear PtBA-peptide conjugates as a vaccine-delivery system against Group A Streptococcus. Materials & methods: PtBA nanoparticles bearing J14 peptide epitopes were prepared via alkyne-azide 1,3-dipolar cycloaddition 'click reaction. The conjugated products were self-assembled into small or large nanoparticles. These nanoparticle vaccine candidates were evaluated in vivo and J14-specific antibody titers were assessed. Results & discussion: Mice vaccinated with the nanoparticles were able to produce J14-specific IgG antibodies without the use of an external adjuvant after a single immunization. We have demonstrated for the first time that the immune responses against self-assembled PtBA nanoparticles are stronger for the smaller sized {\~(}20 nm) nanoparticles compared with the larger {\~(}500nm) PtBA nanoparticles. Conclusion: PtBA analogs have the potential to be developed as potent carrier systems for single-dose synthetic vaccines.",

keywords = "nanoparticle, particle size, peptide-based subunit vaccine, poly(t-butyl)acrylate, self-adjuvanting vaccine, single immunization, Streptococcus pyogenes, vaccine delivery, vaccine formulation",

author = "{Ahmad Fuaad}, {Abdullah Ah} and Zhongfan Jia and Mehfuz Zaman and Jon Hartas and Ziora, {Zyta M.} and Lin, {I. Chun} and Moyle, {Peter M.} and Batzloff, {Michael R.} and Good, {Michael F.} and Monteiro, {Michael J.} and Mariusz Skwarczynski and Istvan Toth",

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doi = "10.2217/nnm.13.7",

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AU - Ahmad Fuaad, Abdullah Ah

AU - Jia, Zhongfan

AU - Zaman, Mehfuz

AU - Hartas, Jon

AU - Ziora, Zyta M.

AU - Lin, I. Chun

AU - Moyle, Peter M.

AU - Batzloff, Michael R.

AU - Good, Michael F.

AU - Monteiro, Michael J.

AU - Skwarczynski, Mariusz

AU - Toth, Istvan

PY - 2013/12/19

Y1 - 2013/12/19

N2 - Aim: To explore four-arm star poly(t-butyl)acrylate (PtBA)- peptide and linear PtBA-peptide conjugates as a vaccine-delivery system against Group A Streptococcus. Materials & methods: PtBA nanoparticles bearing J14 peptide epitopes were prepared via alkyne-azide 1,3-dipolar cycloaddition 'click reaction. The conjugated products were self-assembled into small or large nanoparticles. These nanoparticle vaccine candidates were evaluated in vivo and J14-specific antibody titers were assessed. Results & discussion: Mice vaccinated with the nanoparticles were able to produce J14-specific IgG antibodies without the use of an external adjuvant after a single immunization. We have demonstrated for the first time that the immune responses against self-assembled PtBA nanoparticles are stronger for the smaller sized (̃20 nm) nanoparticles compared with the larger (̃500nm) PtBA nanoparticles. Conclusion: PtBA analogs have the potential to be developed as potent carrier systems for single-dose synthetic vaccines.

AB - Aim: To explore four-arm star poly(t-butyl)acrylate (PtBA)- peptide and linear PtBA-peptide conjugates as a vaccine-delivery system against Group A Streptococcus. Materials & methods: PtBA nanoparticles bearing J14 peptide epitopes were prepared via alkyne-azide 1,3-dipolar cycloaddition 'click reaction. The conjugated products were self-assembled into small or large nanoparticles. These nanoparticle vaccine candidates were evaluated in vivo and J14-specific antibody titers were assessed. Results & discussion: Mice vaccinated with the nanoparticles were able to produce J14-specific IgG antibodies without the use of an external adjuvant after a single immunization. We have demonstrated for the first time that the immune responses against self-assembled PtBA nanoparticles are stronger for the smaller sized (̃20 nm) nanoparticles compared with the larger (̃500nm) PtBA nanoparticles. Conclusion: PtBA analogs have the potential to be developed as potent carrier systems for single-dose synthetic vaccines.

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KW - particle size

KW - peptide-based subunit vaccine

KW - poly(t-butyl)acrylate

KW - self-adjuvanting vaccine

KW - single immunization

KW - Streptococcus pyogenes

KW - vaccine delivery

KW - vaccine formulation

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Polymer-peptide hybrids as a highly immunogenic single-dose nanovaccine

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Keywords

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