Porous silicon-based nanostructured microparticles as degradable supports for solid-phase synthesis and release of oligonucleotides

Steven McInnes, Nicolas Voelcker

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    We describe the preparation of several types of porous silicon (pSi) microparticles as supports for the solid-phase synthesis of oligonucleotides. The first of these supports facilitates oligonucleotide release from the nanostructured support during the oligonucleotide deprotection step, While the second two types of supports are able to withstand the cleavage and deprotection of the oligonucleotides post synthesis and subsequently dissolve at physiological conditions (pH = 7.4, 37 °C), Slowly releasing the oligonucleotides. Our approach involves the fabrication of pSi microparticles and their functionalisation via hydrosilylation reactions to generate a dimethoxytrityl-protected alcohol on the pSi surface as an initiation point for the synthesis of short oligonucleotides.

    Original languageEnglish
    Article number385
    Number of pages10
    JournalNanoscale Research Letters
    Volume7
    Issue number385
    DOIs
    Publication statusPublished - 2012

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