TY - JOUR
T1 - Portable Colorimetric Device with Commercial Microplates for Quantitative Detection of Urine Biomarkers
T2 - Design, Development, and Clinical Evaluation
AU - Pham, Anh Tran Tam
AU - Tohl, Damian
AU - Hu, Qi
AU - Li, Jordan
AU - Reynolds, Karen J.
AU - Tang, Youhong
PY - 2022/9
Y1 - 2022/9
N2 - Urine biomarkers are important in monitoring diseases related to human kidney function. The current processes for measuring biomarker levels in urine samples require patients to regularly visit clinical facilities, which is inconvenient and sometimes impossible for patients in rural areas. Therefore, portable analysis devices for the measurement of urine biomarkers are urgently requested. In this study, a portable platform using colorimetry, a common and simple-to-operate chemical analysis technique, was developed to measure urine biomarkers. The device, using commercial test kits as recognising reagents and a 96-well microplate as a solution container, provides quantitative measures of biomarker concentration. Moreover, the proposed device introduces a calibration method to minimise the dependence of regular maintenance. The device's performance was evaluated with urine from 73 renal patients and its results matched with clinical results well. The device has the potential for measuring urine creatinine, in addition to performing a variety of commercial assays for biomarker detection in human body fluids in general.
AB - Urine biomarkers are important in monitoring diseases related to human kidney function. The current processes for measuring biomarker levels in urine samples require patients to regularly visit clinical facilities, which is inconvenient and sometimes impossible for patients in rural areas. Therefore, portable analysis devices for the measurement of urine biomarkers are urgently requested. In this study, a portable platform using colorimetry, a common and simple-to-operate chemical analysis technique, was developed to measure urine biomarkers. The device, using commercial test kits as recognising reagents and a 96-well microplate as a solution container, provides quantitative measures of biomarker concentration. Moreover, the proposed device introduces a calibration method to minimise the dependence of regular maintenance. The device's performance was evaluated with urine from 73 renal patients and its results matched with clinical results well. The device has the potential for measuring urine creatinine, in addition to performing a variety of commercial assays for biomarker detection in human body fluids in general.
KW - colorimetric sensing
KW - urine biomarkers
KW - portable medical device
KW - clinical evaluation
KW - rural areas
U2 - 10.3390/bios12090723
DO - 10.3390/bios12090723
M3 - Article
SN - 2079-6374
VL - 12
JO - Biosensors
JF - Biosensors
IS - 9
M1 - 723
ER -
