Potassium conductance increased by noradrenaline, opioids, somatostatin, and G-proteins: Whole-cell recording from guinea pig submucous neurons

H. Tatsumi, M. Costa, M. Schimerlik, R. A. North

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65 Citations (Scopus)

Abstract


Agonists at α2-adrenoceptors, δ-opioid receptors, and somatostatin receptors were applied to dissociated guinea pig submucous plexus neurons; whole-cell recordings of membrane current showed that they increased the membrane potassium conductance. The conductance affected showed inward rectification, being described by Gag(max)/{1 + exp((V - V0.5)/k)} where V0.5 was about -65 mV and Gag(max) was about 10 nS. The agonists were ineffective when the potassium conductance of the neurons had first been increased by intracellular dialysis with purified guanosine 5′-triphosphate (GTP)-binding proteins (Gi or Go). Agonist actions were prevented by pertussis toxin, applied intracellularly (10-100 ng/ml for several minutes) or extracellularly 1-10 μg/ml for 1 hr); in the latter case, the agonist responses were reconstituted by intracellular dialysis with GTP-binding proteins.

Original languageEnglish
Pages (from-to)1675-1682
Number of pages8
JournalJournal of Neuroscience
Volume10
Issue number5
DOIs
Publication statusPublished - 1 May 1990
Externally publishedYes

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