Potential translation of plasma microRNA signatures in the risk stratification of COVID-19 patients admitted to the ICU

Iván Benitez; Amara Carratalá; Anna Moncusí-Moix; Clara Gort-Paniello; Lucia Pinilla; Marta Molinero; Jessica González; Gerard Torres; Maria Bernal; Silvia Pico; Raquel Almansa; Noelia Jorge; Alicia Ortega; Elena Bustamante-Munguira; Jose Manuel Gomez; Milagros González-Rivera; Dariela Micheloud; Pablo Ryan; Amalia Martinez; Luis Tamayo; César  Aldecoa; Ricard Ferrer; Adrián Ceccato; Laia Fernández; Ana Motos; Jordi Riera; Rosario Menéndez; Dario Garcia-Gasulla; Oscar Penuelas; Antoni Torres; Jesús F. Bermejo-Martin; Ferran Barbé; David de Gonzalo Calvo

doi:10.1183/13993003.congress-2021.PA666

Potential translation of plasma microRNA signatures in the risk stratification of COVID-19 patients admitted to the ICU

Iván Benitez, Amara Carratalá, Anna Moncusí-Moix, Clara Gort-Paniello, Lucia Pinilla, Marta Molinero, Jessica González, Gerard Torres, Maria Bernal, Silvia Pico, Raquel Almansa, Noelia Jorge, Alicia Ortega, Elena Bustamante-Munguira, Jose Manuel Gomez, Milagros González-Rivera, Dariela Micheloud, Pablo Ryan, Amalia Martinez, Luis TamayoCésar Aldecoa, Ricard Ferrer, Adrián Ceccato, Laia Fernández, Ana Motos, Jordi Riera, Rosario Menéndez, Dario Garcia-Gasulla, Oscar Penuelas, Antoni Torres, Jesús F. Bermejo-Martin, Ferran Barbé, David de Gonzalo Calvo

Research output: Contribution to journal › Meeting Abstract › peer-review

Abstract

Background: The investigation clinical indicators provided by emerging omics technologies may shed light on the development of novel tools for the management of COVID-19.

Aim: to explore the potential role and clinical significance of the plasma microRNA (miRNA) profile as a source of biomarkers of disease severity.

Methods: Observational and multicenter study. Eighty-four patients with a positive for SARS-CoV-2 recruited during the first pandemic wave in Spain were included. The patients were divided according to the severity of the disease into the following groups: i) hospitalized patients without requiring critical care, and ii) hospitalized patients admitted to the ICU. An additional study including ICU nonsurvivors and survivors was performed. Plasma miRNA profiling was analyzed using RT-qPCR.

Results: Ten miRNAs were dysregulated in ICU patients compared to ward patients. The multivariable analysis identified a signature of three miRNAs (miR-148a-3p, miR-451a and miR-486-5p) that displayed an optimal discriminatory ability to distinguish between ICU and ward patients (AUC=0.89). Among critically ill patients, six miRNAs showed significant suppression in patients who did not survive the ICU stay. The multivariable analysis selected a signature based on two miRNAs, miR-192-5p and miR-323a-3p, that was a relevant predictor of mortality during the ICU stay (AUC = 0.80). The discriminatory potential of the miRNA signature was higher than that observed for clinical laboratory parameters such as CRP, D-dimer or leukocyte counts (maximum AUC=0.73).

Conclusions: Plasma miRNA signatures may be useful for risk-based patient stratification over current tests.

Original language	English
Article number	PA666
Number of pages	1
Journal	European Respiratory Journal
Volume	58
Issue number	Supplement 65
DOIs	https://doi.org/10.1183/13993003.congress-2021.PA666
Publication status	Published - 2021
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

COVID-19
management
plasma miRNA

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Benitez, I., Carratalá, A., Moncusí-Moix, A., Gort-Paniello, C., Pinilla, L., Molinero, M., González, J., Torres, G., Bernal, M., Pico, S., Almansa, R., Jorge, N., Ortega, A., Bustamante-Munguira, E., Gomez, J. M., González-Rivera, M., Micheloud, D., Ryan, P., Martinez, A., ... de Gonzalo Calvo, D. (2021). Potential translation of plasma microRNA signatures in the risk stratification of COVID-19 patients admitted to the ICU. European Respiratory Journal, 58(Supplement 65), Article PA666. https://doi.org/10.1183/13993003.congress-2021.PA666

@article{b125adbcd1cd4803891d6a6414449001,

title = "Potential translation of plasma microRNA signatures in the risk stratification of COVID-19 patients admitted to the ICU",

abstract = "Background: The investigation clinical indicators provided by emerging omics technologies may shed light on the development of novel tools for the management of COVID-19.Aim: to explore the potential role and clinical significance of the plasma microRNA (miRNA) profile as a source of biomarkers of disease severity.Methods: Observational and multicenter study. Eighty-four patients with a positive for SARS-CoV-2 recruited during the first pandemic wave in Spain were included. The patients were divided according to the severity of the disease into the following groups: i) hospitalized patients without requiring critical care, and ii) hospitalized patients admitted to the ICU. An additional study including ICU nonsurvivors and survivors was performed. Plasma miRNA profiling was analyzed using RT-qPCR.Results: Ten miRNAs were dysregulated in ICU patients compared to ward patients. The multivariable analysis identified a signature of three miRNAs (miR-148a-3p, miR-451a and miR-486-5p) that displayed an optimal discriminatory ability to distinguish between ICU and ward patients (AUC=0.89). Among critically ill patients, six miRNAs showed significant suppression in patients who did not survive the ICU stay. The multivariable analysis selected a signature based on two miRNAs, miR-192-5p and miR-323a-3p, that was a relevant predictor of mortality during the ICU stay (AUC = 0.80). The discriminatory potential of the miRNA signature was higher than that observed for clinical laboratory parameters such as CRP, D-dimer or leukocyte counts (maximum AUC=0.73).Conclusions: Plasma miRNA signatures may be useful for risk-based patient stratification over current tests.",

keywords = "COVID-19, management, plasma miRNA",

author = "Iv{\'a}n Benitez and Amara Carratal{\'a} and Anna Moncus{\'i}-Moix and Clara Gort-Paniello and Lucia Pinilla and Marta Molinero and Jessica Gonz{\'a}lez and Gerard Torres and Maria Bernal and Silvia Pico and Raquel Almansa and Noelia Jorge and Alicia Ortega and Elena Bustamante-Munguira and Gomez, {Jose Manuel} and Milagros Gonz{\'a}lez-Rivera and Dariela Micheloud and Pablo Ryan and Amalia Martinez and Luis Tamayo and C{\'e}sar Aldecoa and Ricard Ferrer and Adri{\'a}n Ceccato and Laia Fern{\'a}ndez and Ana Motos and Jordi Riera and Rosario Men{\'e}ndez and Dario Garcia-Gasulla and Oscar Penuelas and Antoni Torres and Bermejo-Martin, {Jes{\'u}s F.} and Ferran Barb{\'e} and {de Gonzalo Calvo}, David",

year = "2021",

doi = "10.1183/13993003.congress-2021.PA666",

language = "English",

volume = "58",

journal = "European Respiratory Journal",

issn = "1399-3003",

publisher = "European Respiratory Society",

number = "Supplement 65",

}

Benitez, I, Carratalá, A, Moncusí-Moix, A, Gort-Paniello, C, Pinilla, L, Molinero, M, González, J, Torres, G, Bernal, M, Pico, S, Almansa, R, Jorge, N, Ortega, A, Bustamante-Munguira, E, Gomez, JM, González-Rivera, M, Micheloud, D, Ryan, P, Martinez, A, Tamayo, L, Aldecoa, C, Ferrer, R, Ceccato, A, Fernández, L, Motos, A, Riera, J, Menéndez, R, Garcia-Gasulla, D, Penuelas, O, Torres, A, Bermejo-Martin, JF, Barbé, F & de Gonzalo Calvo, D 2021, 'Potential translation of plasma microRNA signatures in the risk stratification of COVID-19 patients admitted to the ICU', European Respiratory Journal, vol. 58, no. Supplement 65, PA666. https://doi.org/10.1183/13993003.congress-2021.PA666

TY - JOUR

T1 - Potential translation of plasma microRNA signatures in the risk stratification of COVID-19 patients admitted to the ICU

AU - Benitez, Iván

AU - Carratalá, Amara

AU - Moncusí-Moix, Anna

AU - Gort-Paniello, Clara

AU - Pinilla, Lucia

AU - Molinero, Marta

AU - González, Jessica

AU - Torres, Gerard

AU - Bernal, Maria

AU - Pico, Silvia

AU - Almansa, Raquel

AU - Jorge, Noelia

AU - Ortega, Alicia

AU - Bustamante-Munguira, Elena

AU - Gomez, Jose Manuel

AU - González-Rivera, Milagros

AU - Micheloud, Dariela

AU - Ryan, Pablo

AU - Martinez, Amalia

AU - Tamayo, Luis

AU - Aldecoa, César

AU - Ferrer, Ricard

AU - Ceccato, Adrián

AU - Fernández, Laia

AU - Motos, Ana

AU - Riera, Jordi

AU - Menéndez, Rosario

AU - Garcia-Gasulla, Dario

AU - Penuelas, Oscar

AU - Torres, Antoni

AU - Bermejo-Martin, Jesús F.

AU - Barbé, Ferran

AU - de Gonzalo Calvo, David

PY - 2021

Y1 - 2021

N2 - Background: The investigation clinical indicators provided by emerging omics technologies may shed light on the development of novel tools for the management of COVID-19.Aim: to explore the potential role and clinical significance of the plasma microRNA (miRNA) profile as a source of biomarkers of disease severity.Methods: Observational and multicenter study. Eighty-four patients with a positive for SARS-CoV-2 recruited during the first pandemic wave in Spain were included. The patients were divided according to the severity of the disease into the following groups: i) hospitalized patients without requiring critical care, and ii) hospitalized patients admitted to the ICU. An additional study including ICU nonsurvivors and survivors was performed. Plasma miRNA profiling was analyzed using RT-qPCR.Results: Ten miRNAs were dysregulated in ICU patients compared to ward patients. The multivariable analysis identified a signature of three miRNAs (miR-148a-3p, miR-451a and miR-486-5p) that displayed an optimal discriminatory ability to distinguish between ICU and ward patients (AUC=0.89). Among critically ill patients, six miRNAs showed significant suppression in patients who did not survive the ICU stay. The multivariable analysis selected a signature based on two miRNAs, miR-192-5p and miR-323a-3p, that was a relevant predictor of mortality during the ICU stay (AUC = 0.80). The discriminatory potential of the miRNA signature was higher than that observed for clinical laboratory parameters such as CRP, D-dimer or leukocyte counts (maximum AUC=0.73).Conclusions: Plasma miRNA signatures may be useful for risk-based patient stratification over current tests.

AB - Background: The investigation clinical indicators provided by emerging omics technologies may shed light on the development of novel tools for the management of COVID-19.Aim: to explore the potential role and clinical significance of the plasma microRNA (miRNA) profile as a source of biomarkers of disease severity.Methods: Observational and multicenter study. Eighty-four patients with a positive for SARS-CoV-2 recruited during the first pandemic wave in Spain were included. The patients were divided according to the severity of the disease into the following groups: i) hospitalized patients without requiring critical care, and ii) hospitalized patients admitted to the ICU. An additional study including ICU nonsurvivors and survivors was performed. Plasma miRNA profiling was analyzed using RT-qPCR.Results: Ten miRNAs were dysregulated in ICU patients compared to ward patients. The multivariable analysis identified a signature of three miRNAs (miR-148a-3p, miR-451a and miR-486-5p) that displayed an optimal discriminatory ability to distinguish between ICU and ward patients (AUC=0.89). Among critically ill patients, six miRNAs showed significant suppression in patients who did not survive the ICU stay. The multivariable analysis selected a signature based on two miRNAs, miR-192-5p and miR-323a-3p, that was a relevant predictor of mortality during the ICU stay (AUC = 0.80). The discriminatory potential of the miRNA signature was higher than that observed for clinical laboratory parameters such as CRP, D-dimer or leukocyte counts (maximum AUC=0.73).Conclusions: Plasma miRNA signatures may be useful for risk-based patient stratification over current tests.

KW - COVID-19

KW - management

KW - plasma miRNA

U2 - 10.1183/13993003.congress-2021.PA666

DO - 10.1183/13993003.congress-2021.PA666

M3 - Meeting Abstract

SN - 1399-3003

VL - 58

JO - European Respiratory Journal

JF - European Respiratory Journal

IS - Supplement 65

M1 - PA666

ER -

Potential translation of plasma microRNA signatures in the risk stratification of COVID-19 patients admitted to the ICU

Abstract

UN SDGs

Keywords

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