Potential value of the Limulus lysate assay for the measurement of meat spoilage

Using a microtitre plate method the Limulus lysate endotoxin assay was shown to be capable of detecting Pseudomonas spp. at viable cell concentrations of 10²‐l0³/ml. The changes in endotoxin concentration, microbial flora, pH and ERV of laboratory‐produced minced beef and minced pork stored at 4°C were monitored. The endotoxin concentration was shown to increase with the onset of spoilage. Factors were derived enabling an estimate of AMC to be made from the endotoxin concentration determined by the Limulus lysate assay. The use of the microtitre plate system significantly reduces the cost of the Limulus lysate assay.