PPAR-alpha cloning, expression, and characterization

Suong Ngo; Ross McKinnon

doi:10.1007/978-1-62703-155-4_2

PPAR-alpha cloning, expression, and characterization

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays an important role in the regulation of many genes involved in key metabolic processes. Today, PPARα has been cloned from mammalian, marsupial, and a number of marine species and its expression has been found to be relatively tissue- and species-specific. Here, we describe the methods for cloning of PPARα genes by RT-PCR and RACE approaches and related protocols for studying the expression of cloned PPARα cDNAs in mammalian cell systems.

Original language	English
Title of host publication	Peroxisome Proliferator-Activated Receptors (PPARs)
Subtitle of host publication	Methods and Protocols
Editors	Mostafa Badr, Jihan Youssef
Publisher	HUMANA PRESS
Pages	7-34
Number of pages	28
ISBN (Print)	9781627031547
DOIs	https://doi.org/10.1007/978-1-62703-155-4_2
Publication status	Published - 2013

Publication series

Name	Methods in Molecular Biology
Volume	952
ISSN (Print)	1064-3745

Keywords

Cos-7 cell expression system
Immunoblots
PPARα cloning
RACE
RT-PCR

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10.1007/978-1-62703-155-4_2

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AB - Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays an important role in the regulation of many genes involved in key metabolic processes. Today, PPARα has been cloned from mammalian, marsupial, and a number of marine species and its expression has been found to be relatively tissue- and species-specific. Here, we describe the methods for cloning of PPARα genes by RT-PCR and RACE approaches and related protocols for studying the expression of cloned PPARα cDNAs in mammalian cell systems.

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KW - Immunoblots

KW - PPARα cloning

KW - RACE

KW - RT-PCR

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PPAR-alpha cloning, expression, and characterization

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