@inbook{84577a9e880e46039ed341034fd7c3e7,
title = "PPAR-alpha cloning, expression, and characterization",
abstract = "Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays an important role in the regulation of many genes involved in key metabolic processes. Today, PPARα has been cloned from mammalian, marsupial, and a number of marine species and its expression has been found to be relatively tissue- and species-specific. Here, we describe the methods for cloning of PPARα genes by RT-PCR and RACE approaches and related protocols for studying the expression of cloned PPARα cDNAs in mammalian cell systems.",
keywords = "Cos-7 cell expression system, Immunoblots, PPARα cloning, RACE, RT-PCR",
author = "Suong Ngo and Ross McKinnon",
year = "2013",
doi = "10.1007/978-1-62703-155-4_2",
language = "English",
isbn = "9781627031547",
series = "Methods in Molecular Biology",
publisher = "HUMANA PRESS",
pages = "7--34",
editor = "Mostafa Badr and Jihan Youssef",
booktitle = "Peroxisome Proliferator-Activated Receptors (PPARs)",
}