PPAR-alpha cloning, expression, and characterization

Suong Ngo; Ross McKinnon

doi:10.1007/978-1-62703-155-4_2

PPAR-alpha cloning, expression, and characterization

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays an important role in the regulation of many genes involved in key metabolic processes. Today, PPARα has been cloned from mammalian, marsupial, and a number of marine species and its expression has been found to be relatively tissue- and species-specific. Here, we describe the methods for cloning of PPARα genes by RT-PCR and RACE approaches and related protocols for studying the expression of cloned PPARα cDNAs in mammalian cell systems.

Original language	English
Title of host publication	Peroxisome Proliferator-Activated Receptors (PPARs)
Subtitle of host publication	Methods and Protocols
Editors	Mostafa Badr, Jihan Youssef
Publisher	HUMANA PRESS
Pages	7-34
Number of pages	28
ISBN (Print)	9781627031547
DOIs	https://doi.org/10.1007/978-1-62703-155-4_2
Publication status	Published - 2013

Publication series

Name	Methods in Molecular Biology
Volume	952
ISSN (Print)	1064-3745

Keywords

Cos-7 cell expression system
Immunoblots
PPARα cloning
RACE
RT-PCR

Access to Document

10.1007/978-1-62703-155-4_2

Fingerprint Dive into the research topics of 'PPAR-alpha cloning, expression, and characterization'. Together they form a unique fingerprint.

Cite this

@inbook{84577a9e880e46039ed341034fd7c3e7,

title = "PPAR-alpha cloning, expression, and characterization",

abstract = "Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays an important role in the regulation of many genes involved in key metabolic processes. Today, PPARα has been cloned from mammalian, marsupial, and a number of marine species and its expression has been found to be relatively tissue- and species-specific. Here, we describe the methods for cloning of PPARα genes by RT-PCR and RACE approaches and related protocols for studying the expression of cloned PPARα cDNAs in mammalian cell systems.",

keywords = "Cos-7 cell expression system, Immunoblots, PPARα cloning, RACE, RT-PCR",

author = "Suong Ngo and Ross McKinnon",

year = "2013",

doi = "10.1007/978-1-62703-155-4_2",

language = "English",

isbn = "9781627031547",

series = "Methods in Molecular Biology",

publisher = "HUMANA PRESS",

pages = "7--34",

editor = "Mostafa Badr and Jihan Youssef",

booktitle = "Peroxisome Proliferator-Activated Receptors (PPARs)",

}

TY - CHAP

T1 - PPAR-alpha cloning, expression, and characterization

AU - Ngo, Suong

AU - McKinnon, Ross

PY - 2013

Y1 - 2013

N2 - Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays an important role in the regulation of many genes involved in key metabolic processes. Today, PPARα has been cloned from mammalian, marsupial, and a number of marine species and its expression has been found to be relatively tissue- and species-specific. Here, we describe the methods for cloning of PPARα genes by RT-PCR and RACE approaches and related protocols for studying the expression of cloned PPARα cDNAs in mammalian cell systems.

AB - Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays an important role in the regulation of many genes involved in key metabolic processes. Today, PPARα has been cloned from mammalian, marsupial, and a number of marine species and its expression has been found to be relatively tissue- and species-specific. Here, we describe the methods for cloning of PPARα genes by RT-PCR and RACE approaches and related protocols for studying the expression of cloned PPARα cDNAs in mammalian cell systems.

KW - Cos-7 cell expression system

KW - Immunoblots

KW - PPARα cloning

KW - RACE

KW - RT-PCR

UR - http://www.scopus.com/inward/record.url?scp=84870539734&partnerID=8YFLogxK

U2 - 10.1007/978-1-62703-155-4_2

DO - 10.1007/978-1-62703-155-4_2

M3 - Chapter

SN - 9781627031547

T3 - Methods in Molecular Biology

SP - 7

EP - 34

BT - Peroxisome Proliferator-Activated Receptors (PPARs)

A2 - Badr, Mostafa

A2 - Youssef, Jihan

PB - HUMANA PRESS

ER -

PPAR-alpha cloning, expression, and characterization

Abstract

Publication series

Keywords

Access to Document

Other files and links

Cite this