PPAR-alpha cloning, expression, and characterization

Suong Ngo, Ross McKinnon

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays an important role in the regulation of many genes involved in key metabolic processes. Today, PPARα has been cloned from mammalian, marsupial, and a number of marine species and its expression has been found to be relatively tissue- and species-specific. Here, we describe the methods for cloning of PPARα genes by RT-PCR and RACE approaches and related protocols for studying the expression of cloned PPARα cDNAs in mammalian cell systems.

    Original languageEnglish
    Title of host publicationPeroxisome Proliferator-Activated Receptors (PPARs)
    Subtitle of host publicationMethods and Protocols
    EditorsMostafa Badr, Jihan Youssef
    PublisherHUMANA PRESS
    Pages7-34
    Number of pages28
    ISBN (Print)9781627031547
    DOIs
    Publication statusPublished - 2013

    Publication series

    NameMethods in Molecular Biology
    Volume952
    ISSN (Print)1064-3745

    Keywords

    • Cos-7 cell expression system
    • Immunoblots
    • PPARα cloning
    • RACE
    • RT-PCR

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