Preliminary studies on scoring micronuclei by computerised image analysis

M. Fenech; L. R. Jarvis; A. A. Morley

doi:10.1016/0165-1161(88)90005-2

Preliminary studies on scoring micronuclei by computerised image analysis

M. Fenech, L. R. Jarvis, A. A. Morley

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25 Citations (Scopus)

Abstract

Initial studies of the use of computerised image analysis to determine micronucleus frequencies in human lymphocytes that have completed one nuclear division are described. Two methods, based on (a) bromodeoxyuridine incorporation and (b) cytokinesis blocking with cytochalasin-B, were studied. The former method is directly amenable to automation. Cytokinesis-blocked cells could not be automatically recognised by image analysis but it was possible to obtain the correct micronucleus frequency from the integrated optical density histograms by using the mononucleate/binucleate cell ratio obtained by visual analysis. The mean (±1 S.E.) integrated optical density of X-ray-induced micronuclei was 11.2% (±1.1) of that measured for nuclei of G₁ cells.

Original language	English
Pages (from-to)	33-38
Number of pages	6
Journal	Mutation Research/Environmental Mutagenesis and Related Subjects
Volume	203
Issue number	1
DOIs	https://doi.org/10.1016/0165-1161(88)90005-2
Publication status	Published - Feb 1988
Externally published	Yes

Keywords

Computerized image analysis
Micronucleus frequency determination

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title = "Preliminary studies on scoring micronuclei by computerised image analysis",

abstract = "Initial studies of the use of computerised image analysis to determine micronucleus frequencies in human lymphocytes that have completed one nuclear division are described. Two methods, based on (a) bromodeoxyuridine incorporation and (b) cytokinesis blocking with cytochalasin-B, were studied. The former method is directly amenable to automation. Cytokinesis-blocked cells could not be automatically recognised by image analysis but it was possible to obtain the correct micronucleus frequency from the integrated optical density histograms by using the mononucleate/binucleate cell ratio obtained by visual analysis. The mean (±1 S.E.) integrated optical density of X-ray-induced micronuclei was 11.2% (±1.1) of that measured for nuclei of G1 cells.",

keywords = "Computerized image analysis, Micronucleus frequency determination",

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year = "1988",

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T1 - Preliminary studies on scoring micronuclei by computerised image analysis

AU - Fenech, M.

AU - Jarvis, L. R.

AU - Morley, A. A.

PY - 1988/2

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N2 - Initial studies of the use of computerised image analysis to determine micronucleus frequencies in human lymphocytes that have completed one nuclear division are described. Two methods, based on (a) bromodeoxyuridine incorporation and (b) cytokinesis blocking with cytochalasin-B, were studied. The former method is directly amenable to automation. Cytokinesis-blocked cells could not be automatically recognised by image analysis but it was possible to obtain the correct micronucleus frequency from the integrated optical density histograms by using the mononucleate/binucleate cell ratio obtained by visual analysis. The mean (±1 S.E.) integrated optical density of X-ray-induced micronuclei was 11.2% (±1.1) of that measured for nuclei of G1 cells.

AB - Initial studies of the use of computerised image analysis to determine micronucleus frequencies in human lymphocytes that have completed one nuclear division are described. Two methods, based on (a) bromodeoxyuridine incorporation and (b) cytokinesis blocking with cytochalasin-B, were studied. The former method is directly amenable to automation. Cytokinesis-blocked cells could not be automatically recognised by image analysis but it was possible to obtain the correct micronucleus frequency from the integrated optical density histograms by using the mononucleate/binucleate cell ratio obtained by visual analysis. The mean (±1 S.E.) integrated optical density of X-ray-induced micronuclei was 11.2% (±1.1) of that measured for nuclei of G1 cells.

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KW - Micronucleus frequency determination

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Preliminary studies on scoring micronuclei by computerised image analysis

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Keywords

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