TY - JOUR
T1 - Preparation and characterization of microcapsules and tablets for probiotic encapsulation via whey protein isolate-nanochitin complex coacervation
AU - Luo, Mengxin
AU - Ma, Le
AU - Guo, Yuxi
AU - Zhu, Caihong
AU - Chen, Junjie
AU - Zhang, Bin
AU - Zhu, Jianguo
AU - Jellicoe, Matt
AU - He, Shan
AU - Zou, Yucong
AU - Yuan, Yang
PY - 2025/1
Y1 - 2025/1
N2 - This research delved into the feasibility of utilizing three nanochitin—chitin nanocrystal (CNC), chitin nanofiber (CNF), and chitin nanosphere (CNS) in complexation with whey protein isolate (WPI) to fabricate complex coacervation and create microcapsules for probiotic encapsulation. The results showed that CNC, CNF, and CNS exhibited notable differences in morphologies, dimensions, and properties due to the respective synthesis methodologies. Nevertheless, all of them maintained a positive charge and were capable of assembling into microcapsules with WPI via electrostatic interactions at optimal pHs. The inclusion of Lactobacillus casei (L. casei) during the complex coacervation phase engendered a shell-like formation around the bacterium within the microcapsule, which enhanced probiotic viability and increased colony-forming unit count. Additionally, these probiotic-loading microcapsules were also processed into tablets, displaying robust structural integrity, augmented protective capabilities, and a distinctive sustained-release profile compared to the microcapsules alone. In summary, this study pioneered the employment of nanochitin formulations in complex coacervation to encapsulate L. casei, spearheading an innovative approach to the creation of a compressed probiotic supplement and contributing to the advancement in the design and fabrication of encapsulation vehicles for active ingredients.
AB - This research delved into the feasibility of utilizing three nanochitin—chitin nanocrystal (CNC), chitin nanofiber (CNF), and chitin nanosphere (CNS) in complexation with whey protein isolate (WPI) to fabricate complex coacervation and create microcapsules for probiotic encapsulation. The results showed that CNC, CNF, and CNS exhibited notable differences in morphologies, dimensions, and properties due to the respective synthesis methodologies. Nevertheless, all of them maintained a positive charge and were capable of assembling into microcapsules with WPI via electrostatic interactions at optimal pHs. The inclusion of Lactobacillus casei (L. casei) during the complex coacervation phase engendered a shell-like formation around the bacterium within the microcapsule, which enhanced probiotic viability and increased colony-forming unit count. Additionally, these probiotic-loading microcapsules were also processed into tablets, displaying robust structural integrity, augmented protective capabilities, and a distinctive sustained-release profile compared to the microcapsules alone. In summary, this study pioneered the employment of nanochitin formulations in complex coacervation to encapsulate L. casei, spearheading an innovative approach to the creation of a compressed probiotic supplement and contributing to the advancement in the design and fabrication of encapsulation vehicles for active ingredients.
KW - Complex coacervation
KW - Microencapsulation
KW - Nanochitin
KW - Probiotic
KW - Whey protein isolate
UR - http://www.scopus.com/inward/record.url?scp=85211000728&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2024.138225
DO - 10.1016/j.ijbiomac.2024.138225
M3 - Article
AN - SCOPUS:85211000728
SN - 0141-8130
VL - 285
JO - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
JF - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
M1 - 138225
ER -