It is often desirable to prepare fresh tissue, without fixation, in order to optimize the label density on fixation-sensitive epitopes. This report describes the use of ethanediol dehydration with the subsequent immunogold localization of a range of epitopes of known distribution, in order to assess the utility of the techniques. The technique fulfills criteria for the retention of both ultrastructure and immunogenicity.
|Number of pages||5|
|Journal||Journal of Computer-Assisted Microscopy|
|Publication status||Published - Mar 1993|
|Event||Joint Conference of the 12th Biennial Conference on Electron Microscopy and the 11th Annual Meeting of the Australia and New Zealand Society for Cell Biology - Perth, Australia|
Duration: 2 Feb 1993 → 2 Feb 1993