Preparation of fresh tissue, without fixation, for ultrastructural immunogold labeling

John W. Stirling, Douglas W. Henderson

Research output: Contribution to journalConference articlepeer-review

Abstract

It is often desirable to prepare fresh tissue, without fixation, in order to optimize the label density on fixation-sensitive epitopes. This report describes the use of ethanediol dehydration with the subsequent immunogold localization of a range of epitopes of known distribution, in order to assess the utility of the techniques. The technique fulfills criteria for the retention of both ultrastructure and immunogenicity.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalJournal of Computer-Assisted Microscopy
Volume5
Issue number1
Publication statusPublished - Mar 1993
Externally publishedYes
EventJoint Conference of the 12th Biennial Conference on Electron Microscopy and the 11th Annual Meeting of the Australia and New Zealand Society for Cell Biology - Perth, Australia
Duration: 2 Feb 19932 Feb 1993

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