Abstract
It is often desirable to prepare fresh tissue, without fixation, in order to optimize the label density on fixation-sensitive epitopes. This report describes the use of ethanediol dehydration with the subsequent immunogold localization of a range of epitopes of known distribution, in order to assess the utility of the techniques. The technique fulfills criteria for the retention of both ultrastructure and immunogenicity.
| Original language | English |
|---|---|
| Pages (from-to) | 1-5 |
| Number of pages | 5 |
| Journal | Journal of Computer-Assisted Microscopy |
| Volume | 5 |
| Issue number | 1 |
| Publication status | Published - Mar 1993 |
| Externally published | Yes |
| Event | Joint Conference of the 12th Biennial Conference on Electron Microscopy and the 11th Annual Meeting of the Australia and New Zealand Society for Cell Biology - Perth, Australia Duration: 2 Feb 1993 → 2 Feb 1993 |