Preparation of fresh tissue, without fixation, for ultrastructural immunogold labeling

John W. Stirling; Douglas W. Henderson

Preparation of fresh tissue, without fixation, for ultrastructural immunogold labeling

John W. Stirling
, Douglas W. Henderson

Research output: Contribution to journal › Conference article › peer-review

Abstract

It is often desirable to prepare fresh tissue, without fixation, in order to optimize the label density on fixation-sensitive epitopes. This report describes the use of ethanediol dehydration with the subsequent immunogold localization of a range of epitopes of known distribution, in order to assess the utility of the techniques. The technique fulfills criteria for the retention of both ultrastructure and immunogenicity.

Original language	English
Pages (from-to)	1-5
Number of pages	5
Journal	Journal of Computer-Assisted Microscopy
Volume	5
Issue number	1
Publication status	Published - Mar 1993
Externally published	Yes
Event	Joint Conference of the 12th Biennial Conference on Electron Microscopy and the 11th Annual Meeting of the Australia and New Zealand Society for Cell Biology - Perth, Australia Duration: 2 Feb 1993 → 2 Feb 1993

Cite this

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title = "Preparation of fresh tissue, without fixation, for ultrastructural immunogold labeling",

abstract = "It is often desirable to prepare fresh tissue, without fixation, in order to optimize the label density on fixation-sensitive epitopes. This report describes the use of ethanediol dehydration with the subsequent immunogold localization of a range of epitopes of known distribution, in order to assess the utility of the techniques. The technique fulfills criteria for the retention of both ultrastructure and immunogenicity.",

author = "Stirling, \{John W.\} and Henderson, \{Douglas W.\}",

year = "1993",

month = mar,

language = "English",

volume = "5",

pages = "1--5",

journal = "Journal of Computer-Assisted Microscopy",

issn = "1040-7286",

publisher = "Plenum Publishers",

number = "1",

note = "Joint Conference of the 12th Biennial Conference on Electron Microscopy and the 11th Annual Meeting of the Australia and New Zealand Society for Cell Biology ; Conference date: 02-02-1993 Through 02-02-1993",

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TY - JOUR

T1 - Preparation of fresh tissue, without fixation, for ultrastructural immunogold labeling

AU - Stirling, John W.

AU - Henderson, Douglas W.

PY - 1993/3

Y1 - 1993/3

N2 - It is often desirable to prepare fresh tissue, without fixation, in order to optimize the label density on fixation-sensitive epitopes. This report describes the use of ethanediol dehydration with the subsequent immunogold localization of a range of epitopes of known distribution, in order to assess the utility of the techniques. The technique fulfills criteria for the retention of both ultrastructure and immunogenicity.

AB - It is often desirable to prepare fresh tissue, without fixation, in order to optimize the label density on fixation-sensitive epitopes. This report describes the use of ethanediol dehydration with the subsequent immunogold localization of a range of epitopes of known distribution, in order to assess the utility of the techniques. The technique fulfills criteria for the retention of both ultrastructure and immunogenicity.

UR - https://www.scopus.com/pages/publications/0027559955

UR - http://www.worldcat.org/oclc/198892542

M3 - Conference article

AN - SCOPUS:0027559955

SN - 1040-7286

VL - 5

SP - 1

EP - 5

JO - Journal of Computer-Assisted Microscopy

JF - Journal of Computer-Assisted Microscopy

IS - 1

T2 - Joint Conference of the 12th Biennial Conference on Electron Microscopy and the 11th Annual Meeting of the Australia and New Zealand Society for Cell Biology

Y2 - 2 February 1993 through 2 February 1993

ER -

Preparation of fresh tissue, without fixation, for ultrastructural immunogold labeling

Abstract

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