Primers for novel microsatellite markers in "fire-specialist" lizards (Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus) and their performance across multiple populations

annabel smith, Michael Gardner, Christopher Bull, Don Driscoll

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    We developed 45 microsatellite markers for three lizard species with fire-related distributions: Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus (17, 12 and 16 markers respectively). To isolate microsatellites we used an enrichment technique for N. stellatus and next-generation sequencing for A. norrisi and C. atlas. Fluorescent tags were attached to primers during PCR for flexible genotyping. All loci were polymorphic with 2-24 alleles and expected heterozygosities of 0.043-0.927. These markers will facilitate studies of post-fire dispersal and recolonisation.

    Original languageEnglish
    Pages (from-to)345-350
    Number of pages6
    JournalConservation Genetics Resources
    Volume3
    Issue number2
    DOIs
    Publication statusPublished - Apr 2011

    Keywords

    • Dispersal
    • Fire ecology
    • Gene flow
    • Population genetics
    • Rept

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