TY - JOUR
T1 - Printable Hydrogel Arrays for Portable and High-Throughput Shear-Mediated Assays
AU - Luo, Xuan
AU - Xing, Wenjin
AU - Delcheva, Iliana
AU - Abdullah Alrashaidi, Fayed
AU - Heydari, Amir
AU - Palms, Dennis
AU - Truong, Vi Khanh
AU - Vasilev, Krasimir
AU - Jia, Zhongfan
AU - Zhang, Wei
AU - Su, Peng
AU - Vimalanathan, Kasturi
AU - Igder, Aghil
AU - Weiss, Gregory A.
AU - Tang, Youhong
AU - MacGregor, Melanie
AU - Raston, Colin L.
PY - 2023/7/5
Y1 - 2023/7/5
N2 - Hydrogels have been widely used to entrap biomolecules for various biocatalytic reactions. However, solute diffusion in these matrices to initiate such reactions can be a very slow process. Conventional mixing remains a challenge as it can cause irreversible distortion or fragmentation of the hydrogel itself. To overcome the diffusion-limit, a shear-stress-mediated platform named the portable vortex-fluidic device (P-VFD) is developed. P-VFD is a portable platform which consists of two main components, (i) a plasma oxazoline-coated polyvinyl chloride (POx-PVC) film with polyacrylamide and alginate (PAAm/Alg-Ca2+) tough hydrogel covalently bound to its surface and (ii) a reactor tube (L × D: 90 mm × 20 mm) where the aforementioned POx-PVC film could be readily inserted for reactions. Through a spotting machine, the PAAm/Alg-Ca2+ hydrogel can be readily printed on a POx-PVC film in an array pattern and up to 25.4 J/m2 adhesion energy can be achieved. The hydrogel arrays on the film not only offer a strong matrix for entrapping biomolecules such as streptavidin-horseradish peroxidase but are also shear stress-tolerant in the reactor tube, enabling a >6-fold increase in its reaction rate after adding tetramethylbenzidine, relative to incubation. Through using the tough hydrogel and its stably bonded substrate, this portable platform effectively overcomes the diffusion-limit and achieves fast assay detection without causing appreciable hydrogel array deformation or dislocation on the substrate film.
AB - Hydrogels have been widely used to entrap biomolecules for various biocatalytic reactions. However, solute diffusion in these matrices to initiate such reactions can be a very slow process. Conventional mixing remains a challenge as it can cause irreversible distortion or fragmentation of the hydrogel itself. To overcome the diffusion-limit, a shear-stress-mediated platform named the portable vortex-fluidic device (P-VFD) is developed. P-VFD is a portable platform which consists of two main components, (i) a plasma oxazoline-coated polyvinyl chloride (POx-PVC) film with polyacrylamide and alginate (PAAm/Alg-Ca2+) tough hydrogel covalently bound to its surface and (ii) a reactor tube (L × D: 90 mm × 20 mm) where the aforementioned POx-PVC film could be readily inserted for reactions. Through a spotting machine, the PAAm/Alg-Ca2+ hydrogel can be readily printed on a POx-PVC film in an array pattern and up to 25.4 J/m2 adhesion energy can be achieved. The hydrogel arrays on the film not only offer a strong matrix for entrapping biomolecules such as streptavidin-horseradish peroxidase but are also shear stress-tolerant in the reactor tube, enabling a >6-fold increase in its reaction rate after adding tetramethylbenzidine, relative to incubation. Through using the tough hydrogel and its stably bonded substrate, this portable platform effectively overcomes the diffusion-limit and achieves fast assay detection without causing appreciable hydrogel array deformation or dislocation on the substrate film.
KW - alginate
KW - assay platform
KW - portable detection device
KW - spotting
KW - tough hydrogel array
UR - http://www.scopus.com/inward/record.url?scp=85164236879&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/ARC/DP200101106
UR - http://purl.org/au-research/grants/ARC/FT200100301
U2 - 10.1021/acsami.3c02353
DO - 10.1021/acsami.3c02353
M3 - Article
C2 - 37339239
AN - SCOPUS:85164236879
SN - 1944-8244
VL - 15
SP - 31114
EP - 31123
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 26
ER -