Probing enzyme-nanoparticle interactions using combinatorial gold nanoparticle libraries

Yanyan Liu, David Alan Winkler, V. Epa, Bin Zhang, Bing Yan

    Research output: Contribution to journalArticlepeer-review

    24 Citations (Scopus)


    The interaction of nanoparticles with proteins is extremely complex, important for understanding the biological properties of nanomaterials, but is very poorly understood. We have employed a combinatorial library of surface modified gold nanoparticles to interrogate the relationships between the nanoparticle surface chemistry and the specific and nonspecific binding to a common, important, and representative enzyme, acetylcholinesterase (AChE). We also used Bayesian neural networks to generate robust quantitative structure-property relationship (QSPR) models relating the nanoparticle surface to the AChE binding that also provided significant understanding into the molecular basis for these interactions. The results illustrate the insights that result from a synergistic blending of experimental combinatorial synthesis and biological testing of nanoparticles with quantitative computational methods and molecular modeling.

    Original languageEnglish
    Pages (from-to)1293-1308
    Number of pages16
    JournalNano Research
    Issue number4
    Publication statusPublished - 1 Apr 2015


    • acetylcholinesterase
    • enzyme inhibition
    • gold nanoparticles
    • modeling
    • neural network
    • protein binding
    • quantitative structure-property relationship (QSPR)
    • surface modification


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