Probing hexaminolevulinate mediated ppix fluorescence in cancer cell suspensions in the presence of chemical adjuvants

Kit Man Chan, Jonathan Gleadle, Krasimir Vasilev, Melanie Macgregor

Research output: Contribution to journalArticle

Abstract

Exogenous administration of hexaminolevulinate (HAL) induces fluorescent protoporphyrin IX (PpIX) accumulation preferentially in cancer cells. However, the PpIX fluorescence intensities between noncancer and cancer cells are highly variable. The contrast between cancer and noncancer cells may be insufficient to reliably discriminate, especially at the single cell level in cancer diagnostics. This study examines the use of the chemical adjuvants dimethylsulphoxide (DMSO) or deferoxamine (DFO) to enhance the HAL induced PpIX accumulation in cancer cells. Our results showed that in some of the incubation conditions tested, the addition of DFO with HAL significantly increased PpIX 21 fluorescence of adherent monolayer cancer cells, but this was never the case for cells in suspension. Permeabilisation with DMSO did not increase PpIX fluorescence. Cell-to-cell interaction may well play an important role in the PpIX accumulation when suspended cells are treated in HAL and adjuvant chemicals.

Original languageEnglish
Article number2963
Number of pages16
JournalInternational Journal of Molecular Sciences
Volume21
Issue number8
DOIs
Publication statusPublished - 22 Apr 2020

Bibliographical note

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Keywords

  • 5-aminolevulinic acid (ALA)
  • Cancer
  • Ferrochelatase
  • Fluorescence
  • Hexaminolevulinate
  • Metabolism
  • Mitochondria
  • Photodynamic
  • Protoporphyrin IX
  • Single cell

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