Probiotic Lactobacillus rhamnosus GG prevents alveolar bone loss in a mouse model of experimental periodontitis

Simona M. Gatej; Victor Marino; Richard Bright; Tracy R. Fitzsimmons; Neville Gully; Peter Zilm; Rachel J. Gibson; Suzanne Edwards; Peter M. Bartold

doi:10.1111/jcpe.12838

Probiotic Lactobacillus rhamnosus GG prevents alveolar bone loss in a mouse model of experimental periodontitis

Simona M. Gatej, Victor Marino, Richard Bright, Tracy R. Fitzsimmons, Neville Gully, Peter Zilm, Rachel J. Gibson, Suzanne Edwards, Peter M. Bartold

Research output: Contribution to journal › Article › peer-review

76 Citations (Scopus)

Abstract

Aim: This study investigated the role of Lactobacillus rhamnosus GG (LGG) on bone loss and local and systemic inflammation in an in vivo mouse model of experimental periodontitis (PD).

Materials and Methods: Experimental PD was induced in mice by oral inoculation with Porphyromonas gingivalis and Fusobacterium nucleatum over a period of 44 days. The probiotic LGG was administered via oral inoculation or oral gavage prior to, and during disease induction. The antimicrobial activity of LGG on the inoculum was also tested. Alveolar bone levels and gingival tissue changes were assessed using in vivo microcomputed tomography and histological analysis. Serum levels of mouse homologues for IL-8 were measured using multiplex assays.

Results: Pre-treatment with probiotics either via oral gavage or via oral inoculation significantly reduced bone loss (p <.0001) and gingival inflammation (p <.0001) when compared with PD group. Oral gavage treatment group had significantly less tartrate-resistant acid phosphatase positive cells (p <.02) then PD group. LGG showed no antimicrobial activity against P. gingivalis and F. nucleatum.

Conclusions: Lactobacillus rhamnosus GG effectively suppresses bone loss in a mouse model of induced PD irrespective of the mode of administration.

Original language	English
Pages (from-to)	204-212
Number of pages	9
Journal	Journal of Clinical Periodontology
Volume	45
Issue number	2
Early online date	9 Nov 2017
DOIs	https://doi.org/10.1111/jcpe.12838
Publication status	Published - Feb 2018
Externally published	Yes

Keywords

alveolar bone loss prevention
inflammation
Lactobacillus rhamnosus GG
periodontitis
probiotic

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title = "Probiotic Lactobacillus rhamnosus GG prevents alveolar bone loss in a mouse model of experimental periodontitis",

abstract = "Aim: This study investigated the role of Lactobacillus rhamnosus GG (LGG) on bone loss and local and systemic inflammation in an in vivo mouse model of experimental periodontitis (PD). Materials and Methods: Experimental PD was induced in mice by oral inoculation with Porphyromonas gingivalis and Fusobacterium nucleatum over a period of 44 days. The probiotic LGG was administered via oral inoculation or oral gavage prior to, and during disease induction. The antimicrobial activity of LGG on the inoculum was also tested. Alveolar bone levels and gingival tissue changes were assessed using in vivo microcomputed tomography and histological analysis. Serum levels of mouse homologues for IL-8 were measured using multiplex assays. Results: Pre-treatment with probiotics either via oral gavage or via oral inoculation significantly reduced bone loss (p <.0001) and gingival inflammation (p <.0001) when compared with PD group. Oral gavage treatment group had significantly less tartrate-resistant acid phosphatase positive cells (p <.02) then PD group. LGG showed no antimicrobial activity against P. gingivalis and F. nucleatum. Conclusions: Lactobacillus rhamnosus GG effectively suppresses bone loss in a mouse model of induced PD irrespective of the mode of administration.",

keywords = "alveolar bone loss prevention, inflammation, Lactobacillus rhamnosus GG, periodontitis, probiotic",

author = "Gatej, {Simona M.} and Victor Marino and Richard Bright and Fitzsimmons, {Tracy R.} and Neville Gully and Peter Zilm and Gibson, {Rachel J.} and Suzanne Edwards and Bartold, {Peter M.}",

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AU - Gatej, Simona M.

AU - Marino, Victor

AU - Bright, Richard

AU - Fitzsimmons, Tracy R.

AU - Gully, Neville

AU - Zilm, Peter

AU - Gibson, Rachel J.

AU - Edwards, Suzanne

AU - Bartold, Peter M.

PY - 2018/2

Y1 - 2018/2

N2 - Aim: This study investigated the role of Lactobacillus rhamnosus GG (LGG) on bone loss and local and systemic inflammation in an in vivo mouse model of experimental periodontitis (PD). Materials and Methods: Experimental PD was induced in mice by oral inoculation with Porphyromonas gingivalis and Fusobacterium nucleatum over a period of 44 days. The probiotic LGG was administered via oral inoculation or oral gavage prior to, and during disease induction. The antimicrobial activity of LGG on the inoculum was also tested. Alveolar bone levels and gingival tissue changes were assessed using in vivo microcomputed tomography and histological analysis. Serum levels of mouse homologues for IL-8 were measured using multiplex assays. Results: Pre-treatment with probiotics either via oral gavage or via oral inoculation significantly reduced bone loss (p <.0001) and gingival inflammation (p <.0001) when compared with PD group. Oral gavage treatment group had significantly less tartrate-resistant acid phosphatase positive cells (p <.02) then PD group. LGG showed no antimicrobial activity against P. gingivalis and F. nucleatum. Conclusions: Lactobacillus rhamnosus GG effectively suppresses bone loss in a mouse model of induced PD irrespective of the mode of administration.

AB - Aim: This study investigated the role of Lactobacillus rhamnosus GG (LGG) on bone loss and local and systemic inflammation in an in vivo mouse model of experimental periodontitis (PD). Materials and Methods: Experimental PD was induced in mice by oral inoculation with Porphyromonas gingivalis and Fusobacterium nucleatum over a period of 44 days. The probiotic LGG was administered via oral inoculation or oral gavage prior to, and during disease induction. The antimicrobial activity of LGG on the inoculum was also tested. Alveolar bone levels and gingival tissue changes were assessed using in vivo microcomputed tomography and histological analysis. Serum levels of mouse homologues for IL-8 were measured using multiplex assays. Results: Pre-treatment with probiotics either via oral gavage or via oral inoculation significantly reduced bone loss (p <.0001) and gingival inflammation (p <.0001) when compared with PD group. Oral gavage treatment group had significantly less tartrate-resistant acid phosphatase positive cells (p <.02) then PD group. LGG showed no antimicrobial activity against P. gingivalis and F. nucleatum. Conclusions: Lactobacillus rhamnosus GG effectively suppresses bone loss in a mouse model of induced PD irrespective of the mode of administration.

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Probiotic Lactobacillus rhamnosus GG prevents alveolar bone loss in a mouse model of experimental periodontitis

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