Production of [14C]Acetylcholine and [14C]Carbon Dioxide from [U–14C]Glucose in Tissue Prisms from Aging Rat Brain

N. R. Sims; K. L. Marek; D. M. Bowen; A. N. Davison

doi:10.1111/j.1471-4159.1982.tb08654.x

Production of [¹⁴C]Acetylcholine and [¹⁴C]Carbon Dioxide from [U–¹⁴C]Glucose in Tissue Prisms from Aging Rat Brain

N. R. Sims, K. L. Marek, D. M. Bowen, A. N. Davison

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

Abstract: Production of [¹⁴C]acetylcholine and ¹⁴CO₂ was examined by using tissue prisms from neocortex, hippocampus, and striatum from rats aged approximately 5 months, 13 months, and 27 months. [¹⁴C]Acetylcholine synthesis in the striatum showed highly significant decreases with age for measurements in the presence of both 5 mm‐ and 31 mm‐K⁺, contrasting with the lack of significant change in ¹⁴CO₂ production in this region. The neocortex and hippocampus showed only small changes, especially when comparison was made between 13‐month and senescent animals. Measurements of the release of [¹⁴C]acetylcholine and influence of atropine on this release confirmed the relative stability with age of the cholinergic system in the neocortex.

Original language	English
Pages (from-to)	488-492
Number of pages	5
Journal	Journal of Neurochemistry
Volume	38
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-4159.1982.tb08654.x
Publication status	Published - Feb 1982
Externally published	Yes

Keywords

Acetylcholine synthesis
Aging
Glucose metabolism

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abstract = "Abstract: Production of [14C]acetylcholine and 14CO2 was examined by using tissue prisms from neocortex, hippocampus, and striatum from rats aged approximately 5 months, 13 months, and 27 months. [14C]Acetylcholine synthesis in the striatum showed highly significant decreases with age for measurements in the presence of both 5 mm‐ and 31 mm‐K+, contrasting with the lack of significant change in 14CO2 production in this region. The neocortex and hippocampus showed only small changes, especially when comparison was made between 13‐month and senescent animals. Measurements of the release of [14C]acetylcholine and influence of atropine on this release confirmed the relative stability with age of the cholinergic system in the neocortex.",

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AU - Bowen, D. M.

AU - Davison, A. N.

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N2 - Abstract: Production of [14C]acetylcholine and 14CO2 was examined by using tissue prisms from neocortex, hippocampus, and striatum from rats aged approximately 5 months, 13 months, and 27 months. [14C]Acetylcholine synthesis in the striatum showed highly significant decreases with age for measurements in the presence of both 5 mm‐ and 31 mm‐K+, contrasting with the lack of significant change in 14CO2 production in this region. The neocortex and hippocampus showed only small changes, especially when comparison was made between 13‐month and senescent animals. Measurements of the release of [14C]acetylcholine and influence of atropine on this release confirmed the relative stability with age of the cholinergic system in the neocortex.

AB - Abstract: Production of [14C]acetylcholine and 14CO2 was examined by using tissue prisms from neocortex, hippocampus, and striatum from rats aged approximately 5 months, 13 months, and 27 months. [14C]Acetylcholine synthesis in the striatum showed highly significant decreases with age for measurements in the presence of both 5 mm‐ and 31 mm‐K+, contrasting with the lack of significant change in 14CO2 production in this region. The neocortex and hippocampus showed only small changes, especially when comparison was made between 13‐month and senescent animals. Measurements of the release of [14C]acetylcholine and influence of atropine on this release confirmed the relative stability with age of the cholinergic system in the neocortex.

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Production of [¹⁴C]Acetylcholine and [¹⁴C]Carbon Dioxide from [U–¹⁴C]Glucose in Tissue Prisms from Aging Rat Brain

Abstract

Keywords

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