Production of two intermediate taxoids, 2-hydroxy- 5α,10β -diacetoxytaxadiene and 2-hydroxy-5α,10β,14β- triacetoxytaxadiene, from Taxus chinensis cell culture

Ming-Bo Gao, Wei Zhang, Xiang-Guo Yue, Hong-Bin Xiao

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    2 Citations (Scopus)

    Abstract

    The production of taxuyunnanine C (Tc) (2α,5α,10β,14β tetraacetoxytaxa-4(20),11(12)-diene) was significantly enhanced when Taxus chinensis cell cultures were treated with methyl jasmonate (MJA) or 2,3-dihydroxypropyl jasmonate (DHPJA), a newly synthesized jasmonate analog, in combination with in situ absorption. Along with the production of Tc, two intermediate taxoids were produced and purified. On the basis of liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) spectral data, the chemical structures of the two intermediate taxoids were found to be 2-hydroxy-5α,10β-diacetoxytaxadiene and 2-hydroxy-5α,10β,14β- triacetoxytaxadiene. This is the first study to report the production of these two taxoids using plant cell culture.

    Original languageEnglish
    Pages (from-to)8486-8491
    Number of pages6
    JournalAfrican Journal of Biotechnology
    Volume9
    Issue number49
    Publication statusPublished - 6 Dec 2010

    Keywords

    • 2,3-dihydroxypropyl jasmonate
    • 2-hydroxy-5α,10β,14β-triacetoxytaxadiene
    • 2-hydroxy-5α,10β-diacetoxytaxadiene
    • In situ absorption
    • Methyl jasmonate
    • Taxus chinensis
    • Taxuyunnanine C

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