Protease-activated alpha-2-macroglobulin can inhibit amyloid formation via two distinct mechanisms

Amy R. Wyatt, Patrick Constantinescu, Heath Ecroyd, Christopher M Dobson, Mark R. Wilson, Janet R. Kumita, Justin J. Yerbury

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

α2-Macroglobulin (α2M) is an extracellular chaperone that inhibits amorphous and fibrillar protein aggregation. The reaction of α2M with proteases results in an 'activated' conformation, where the proteases become covalently-linked within the interior of a cage-like structure formed by α2M. This study investigates, the effect of activation on the ability of α2M to inhibit amyloid formation by Aβ1-42 and I59T human lysozyme and shows that protease-activated α2M can act via two distinct mechanisms: (i) by trapping proteases that remain able to degrade polypeptide chains and (ii) by a chaperone action that prevents misfolded clients from continuing along the amyloid forming pathway. Structured summary of protein interactions: Aβ1-42 and Aβ1-42 bind by fluorescence technology (View interaction)I59T lysozyme and I59T lysozyme bind by light scattering (View interaction)I59T lysozyme and I59T lysozyme bind by fluorescence technology (View interaction)Alpha-lactalbumin and Alpha-lactalbumin bind by fluorescence technology (View interaction)I59T lysozyme and I59T lysozyme bind by electron microscopy (View interaction)Aβ1-42 and Aβ1-42 bind by electron microscopy (View interaction).

Original languageEnglish
Pages (from-to)398-403
Number of pages6
JournalFEBS Letters
Volume587
Issue number5
DOIs
Publication statusPublished - 1 Mar 2013

Keywords

  • α -Macroglobulin
  • Amyloid disease
  • Extracellular chaperone
  • Human lysozyme

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    Wyatt, A. R., Constantinescu, P., Ecroyd, H., Dobson, C. M., Wilson, M. R., Kumita, J. R., & Yerbury, J. J. (2013). Protease-activated alpha-2-macroglobulin can inhibit amyloid formation via two distinct mechanisms. FEBS Letters, 587(5), 398-403. https://doi.org/10.1016/j.febslet.2013.01.020