Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA

Dmitriy Khodakov, Anastasia Khodakova, David Huang, Adrian Linacre, Amanda Ellis

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    Single nucleotide polymorphisms (SNPs) are a prime source of genetic diversity. Discriminating between different SNPs provides an enormous leap towards the better understanding of the uniqueness of biological systems. Here we report on a new approach for SNP discrimination using toehold-mediated DNA strand displacement. The distinctiveness of the approach is based on the combination of both 3- and 4-way branch migration mechanisms, which allows for reliable discrimination of SNPs within double-stranded DNA generated from real-life human mitochondrial DNA samples. Aside from the potential diagnostic value, the current study represents an additional way to control the strand displacement reaction rate without altering other reaction parameters and provides new insights into the influence of single nucleotide substitutions on 3- and 4-way branch migration efficiency and kinetics.

    Original languageEnglish
    Article number8721
    Pages (from-to)Article: 8721
    Number of pages8
    JournalScientific Reports
    Volume5
    DOIs
    Publication statusPublished - 2015

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