Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA

Dmitriy Khodakov; Anastasia Khodakova; David Huang; Adrian Linacre; Amanda Ellis

doi:10.1038/srep08721

Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA

Dmitriy Khodakov, Anastasia Khodakova, David Huang, Adrian Linacre, Amanda Ellis

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Single nucleotide polymorphisms (SNPs) are a prime source of genetic diversity. Discriminating between different SNPs provides an enormous leap towards the better understanding of the uniqueness of biological systems. Here we report on a new approach for SNP discrimination using toehold-mediated DNA strand displacement. The distinctiveness of the approach is based on the combination of both 3- and 4-way branch migration mechanisms, which allows for reliable discrimination of SNPs within double-stranded DNA generated from real-life human mitochondrial DNA samples. Aside from the potential diagnostic value, the current study represents an additional way to control the strand displacement reaction rate without altering other reaction parameters and provides new insights into the influence of single nucleotide substitutions on 3- and 4-way branch migration efficiency and kinetics.

Original language	English
Article number	8721
Pages (from-to)	Article: 8721
Number of pages	8
Journal	Scientific Reports
Volume	5
DOIs	https://doi.org/10.1038/srep08721
Publication status	Published - 2015

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abstract = "Single nucleotide polymorphisms (SNPs) are a prime source of genetic diversity. Discriminating between different SNPs provides an enormous leap towards the better understanding of the uniqueness of biological systems. Here we report on a new approach for SNP discrimination using toehold-mediated DNA strand displacement. The distinctiveness of the approach is based on the combination of both 3- and 4-way branch migration mechanisms, which allows for reliable discrimination of SNPs within double-stranded DNA generated from real-life human mitochondrial DNA samples. Aside from the potential diagnostic value, the current study represents an additional way to control the strand displacement reaction rate without altering other reaction parameters and provides new insights into the influence of single nucleotide substitutions on 3- and 4-way branch migration efficiency and kinetics.",

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AU - Khodakova, Anastasia

AU - Huang, David

AU - Linacre, Adrian

AU - Ellis, Amanda

PY - 2015

Y1 - 2015

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AB - Single nucleotide polymorphisms (SNPs) are a prime source of genetic diversity. Discriminating between different SNPs provides an enormous leap towards the better understanding of the uniqueness of biological systems. Here we report on a new approach for SNP discrimination using toehold-mediated DNA strand displacement. The distinctiveness of the approach is based on the combination of both 3- and 4-way branch migration mechanisms, which allows for reliable discrimination of SNPs within double-stranded DNA generated from real-life human mitochondrial DNA samples. Aside from the potential diagnostic value, the current study represents an additional way to control the strand displacement reaction rate without altering other reaction parameters and provides new insights into the influence of single nucleotide substitutions on 3- and 4-way branch migration efficiency and kinetics.

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SP - Article: 8721

JO - Scientific Reports

JF - Scientific Reports

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ER -

Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA

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