Protective effects of therapeutic hypothermia on renal injury in an asphyxial cardiac arrest rat model

Anowarul Islam, So Eun Kim, Jae Chol Yoon, Ali Jawad, Weishun Tian, Yeo-Jin Yoo, In-Shik Kim, Dongchoon Ahn, Byung-Yong Park, Yong Hwang, Jeong-Ho Lee, Hyun-Jin Tae, Jeong-Hwi Cho, Kyunghwa Kim

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Cardiac arrest (CA) is a leading cause of mortality worldwide. Most of post-resuscitation related deaths are due to post-cardiac arrest syndrome (PCAS). After cardiopulmonary resuscitation (CPR), return of spontaneous circulation (ROSC) leads to renal ischemia-reperfusion injury, also known as PCAS. Many studies have focused on brain and heart injuries after ROSC, but renal failure has largely been ignored. Therefore, we investigated the protective effects of therapeutic hypothermia (TH) on asphyxial CA-induced renal injury in rats.

Thirty rats were randomly divided into five groups: 1) the control group (sham); 2) the normothermic CA (nor.); 3) a normothermic CA group that received TH immediately within 2 h after CPR (Hypo. 2 hrs); 4) a normothermic CA group that received TH within 4 h after CPR (Hypo. 4 hrs); and 5) a normothermia CA group that received TH within 6 h after CPR (Hypo. 6 h). One day after CPR, all rats were sacrificed. Compared with the normothermic CA group, the TH groups demonstrated significantly increased survival rate (P < 0.05); decreased serum blood urea nitrogen, creatinine, and lactate dehydrogenase levels; and lower histological damage degree and malondialdehyde concentration in their renal tissue. Terminal deoxynucleotidyl transferase dUTP nick end labeling stain revealed that the number of apoptotic cells significantly decreased after 4 h and 6 h of TH compared to the results seen in the normothermic CA group. Moreover, TH downregulated the expression of cyclooxygenase-2 in the renal cortex compared to the normothermic CA group one day after CPR. These results suggest that TH exerts anti-apoptotic, anti-inflammatory, and anti-oxidative effects immediately after ROSC that protect against renal injury.
Original languageEnglish
Article number102761
Number of pages7
Early online date13 Oct 2020
Publication statusPublished - Dec 2020
Externally publishedYes


  • Apoptosis
  • Asphyxial cardiac arrest
  • COX-2
  • Renal ischemia-reperfusion injury
  • Therapeutic hypothermia


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