TY - JOUR
T1 - Protein Interactions with Nanoengineered Polyoxazoline Surfaces Generated via Plasma Deposition
AU - Gonzalez Garcia, Laura E.
AU - Macgregor-Ramiasa, Melanie
AU - Visalakshan, Rahul Madathiparambil
AU - Vasilev, Krasimir
PY - 2017/7/25
Y1 - 2017/7/25
N2 - Protein adsorption to biomaterials is critical in determining their suitability for specific applications, such as implants or biosensors. Here, we show that surface nanoroughness can be tailored to control the covalent binding of proteins to plasma-deposited polyoxazoline (PPOx). Nanoengineered surfaces were created by immobilizing gold nanoparticles varying in size and surface density on PPOx films. To keep the surface chemistry consistent while preserving the nanotopography, all substrates were overcoated with a nanothin PPOx film. Bovine serum albumin was chosen to study protein interactions with the nanoengineered surfaces. The results demonstrate that the amount of protein bound to the surface is not directly correlated with the increase in surface area. Instead, it is determined by nanotopography-induced geometric effects and surface wettability. A densely packed array of 16 and 38 nm nanoparticles hinders protein adsorption compared to smooth PPOx substrates, while it increases for 68 nm nanoparticles. These adaptable surfaces could be used for designing biomaterials where proteins adsorption is or is not desirable.
AB - Protein adsorption to biomaterials is critical in determining their suitability for specific applications, such as implants or biosensors. Here, we show that surface nanoroughness can be tailored to control the covalent binding of proteins to plasma-deposited polyoxazoline (PPOx). Nanoengineered surfaces were created by immobilizing gold nanoparticles varying in size and surface density on PPOx films. To keep the surface chemistry consistent while preserving the nanotopography, all substrates were overcoated with a nanothin PPOx film. Bovine serum albumin was chosen to study protein interactions with the nanoengineered surfaces. The results demonstrate that the amount of protein bound to the surface is not directly correlated with the increase in surface area. Instead, it is determined by nanotopography-induced geometric effects and surface wettability. A densely packed array of 16 and 38 nm nanoparticles hinders protein adsorption compared to smooth PPOx substrates, while it increases for 68 nm nanoparticles. These adaptable surfaces could be used for designing biomaterials where proteins adsorption is or is not desirable.
KW - Protein
KW - biomaterials
KW - implants
KW - biosensors
KW - plasma-deposited polyoxazoline
KW - PPOx
KW - Nanoengineered
KW - nanoparticles
KW - PPOx films
KW - nanotopography
KW - nanoroughness
KW - surface
UR - http://www.scopus.com/inward/record.url?scp=85025833997&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/ARC/DP150104212
UR - http://purl.org/au-research/grants/NHMRC/1122825
U2 - 10.1021/acs.langmuir.7b01279
DO - 10.1021/acs.langmuir.7b01279
M3 - Article
C2 - 28658956
AN - SCOPUS:85025833997
VL - 33
SP - 7322
EP - 7331
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 29
ER -