Proteomic analysis of influenza haemagglutinin-specific antibodies following vaccination reveals convergent immunoglobulin variable region signatures

Penelope Adamson; Mahmood Al Kindi; Jing Wang; Alexander Colella; Timothy Chataway; Nikolai Petrovsky; Thomas Gordon; David Gordon

doi:10.1016/j.vaccine.2017.08.053

Proteomic analysis of influenza haemagglutinin-specific antibodies following vaccination reveals convergent immunoglobulin variable region signatures

Penelope Adamson
, Mahmood Al Kindi
, Jing Wang
, Alexander Colella
, Timothy Chataway
, Nikolai Petrovsky
, Thomas Gordon
, David Gordon

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Analysis of the anti-haemagglutinin serum antibody proteome from six H1N1pdm09 influenza A vaccinated subjects demonstrated restricted IgG1 heavy chain species encoded by IGHV5-51 and IGHV3-7 gene families in 2 subjects and either IGHV5-51 or IGHV3-7 in 4 individuals. All subjects exhibited a dominant IGKV3-20 light chain, however 5 subjects also exhibited IGKV3-11 and IGKV4-1 families. Sequences were closely aligned with the matched germline sequence, with few shared mutations. This study illustrates the feasibility of using a proteomic approach to determine the expressed V region signatures of serum antibodies induced by vaccination.

Original language	English
Pages (from-to)	5576-5580
Number of pages	5
Journal	Vaccine
Volume	35
Issue number	42
DOIs	https://doi.org/10.1016/j.vaccine.2017.08.053
Publication status	Published - 9 Oct 2017

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Influenza A
Mass spectrometric sequencing
Proteomics
Serum antibody repertoire
Vaccination

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10.1016/j.vaccine.2017.08.053

Cite this

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title = "Proteomic analysis of influenza haemagglutinin-specific antibodies following vaccination reveals convergent immunoglobulin variable region signatures",

abstract = "Analysis of the anti-haemagglutinin serum antibody proteome from six H1N1pdm09 influenza A vaccinated subjects demonstrated restricted IgG1 heavy chain species encoded by IGHV5-51 and IGHV3-7 gene families in 2 subjects and either IGHV5-51 or IGHV3-7 in 4 individuals. All subjects exhibited a dominant IGKV3-20 light chain, however 5 subjects also exhibited IGKV3-11 and IGKV4-1 families. Sequences were closely aligned with the matched germline sequence, with few shared mutations. This study illustrates the feasibility of using a proteomic approach to determine the expressed V region signatures of serum antibodies induced by vaccination.",

keywords = "Influenza A, Mass spectrometric sequencing, Proteomics, Serum antibody repertoire, Vaccination",

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doi = "10.1016/j.vaccine.2017.08.053",

language = "English",

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T1 - Proteomic analysis of influenza haemagglutinin-specific antibodies following vaccination reveals convergent immunoglobulin variable region signatures

AU - Adamson, Penelope

AU - Al Kindi, Mahmood

AU - Wang, Jing

AU - Colella, Alexander

AU - Chataway, Timothy

AU - Petrovsky, Nikolai

AU - Gordon, Thomas

AU - Gordon, David

PY - 2017/10/9

Y1 - 2017/10/9

N2 - Analysis of the anti-haemagglutinin serum antibody proteome from six H1N1pdm09 influenza A vaccinated subjects demonstrated restricted IgG1 heavy chain species encoded by IGHV5-51 and IGHV3-7 gene families in 2 subjects and either IGHV5-51 or IGHV3-7 in 4 individuals. All subjects exhibited a dominant IGKV3-20 light chain, however 5 subjects also exhibited IGKV3-11 and IGKV4-1 families. Sequences were closely aligned with the matched germline sequence, with few shared mutations. This study illustrates the feasibility of using a proteomic approach to determine the expressed V region signatures of serum antibodies induced by vaccination.

AB - Analysis of the anti-haemagglutinin serum antibody proteome from six H1N1pdm09 influenza A vaccinated subjects demonstrated restricted IgG1 heavy chain species encoded by IGHV5-51 and IGHV3-7 gene families in 2 subjects and either IGHV5-51 or IGHV3-7 in 4 individuals. All subjects exhibited a dominant IGKV3-20 light chain, however 5 subjects also exhibited IGKV3-11 and IGKV4-1 families. Sequences were closely aligned with the matched germline sequence, with few shared mutations. This study illustrates the feasibility of using a proteomic approach to determine the expressed V region signatures of serum antibodies induced by vaccination.

KW - Influenza A

KW - Mass spectrometric sequencing

KW - Proteomics

KW - Serum antibody repertoire

KW - Vaccination

UR - https://www.scopus.com/pages/publications/85028756915

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DO - 10.1016/j.vaccine.2017.08.053

M3 - Article

SN - 0264-410X

VL - 35

SP - 5576

EP - 5580

JO - Vaccine

JF - Vaccine

IS - 42

ER -

Proteomic analysis of influenza haemagglutinin-specific antibodies following vaccination reveals convergent immunoglobulin variable region signatures

Abstract

UN SDGs

Keywords

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