Proteomics investigation of human platelets in healthy donors and cystic fibrosis patients by shotgun nUPLC-MS E and 2DE: A comparative study

Luisa Pieroni, Francesco Finamore, Maurizio Ronci, Domenico Mattoscio, Valeria Marzano, Stefano Mortera, Serena Quattrucci, Giorgio Federico, Mario Romano, Andrea Urbani

    Research output: Contribution to journalArticlepeer-review

    31 Citations (Scopus)

    Abstract

    Platelets are of pathophysiological relevance in haemostasis, wound repair, inflammation and cardiovascular disease. We have shown that human platelets express a biologically active Cystic Fibrosis Transmembrane Conductance Regulator, which is dysfunctional in Cystic Fibrosis (CF) patients, and regulate platelet responses related to inflammation and its resolution. In order to further elucidate platelet involvement in CF inflammation, we pursued a comparative proteomic analysis of cells from healthy donors and CF patients, in association with a non-supervised comparative analysis of the Gene Ontology. Our results, showing changes in the integrin signalling in CF, support a pro-inflammatory profile of CF platelets.

    Original languageEnglish
    Pages (from-to)630-639
    Number of pages10
    JournalMolecular BioSystems
    Volume7
    Issue number3
    DOIs
    Publication statusPublished - 1 Mar 2011

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