Protocol to assess the impact of early-life antibiotic exposure on murine longevity

Miriam A. Lynn; Feargal J. Ryan; Yee C. Tee; Saoirse C. Benson; David J. Lynn

doi:10.1016/j.xpro.2022.101220

Protocol to assess the impact of early-life antibiotic exposure on murine longevity

Miriam A. Lynn, Feargal J. Ryan, Yee C. Tee, Saoirse C. Benson, David J. Lynn

College of Medicine and Public Health

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Abstract

We present this protocol using a mouse model to assess the impact of early-life antibiotic exposure on mammalian lifespan and the composition of the gut microbiota over time. We describe longitudinal fecal sampling and health monitoring following early-life antibiotic exposure. We detail DNA extraction and 16S rRNA gene sequencing to longitudinally profile the composition of the fecal microbiota. Finally, we discuss how to address potential confounders such as the stochastic recolonization of the gut microbiota following antibiotic exposure. For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021).

Original language	English
Article number	101220
Number of pages	22
Journal	STAR Protocols
Volume	3
Issue number	1
Early online date	5 Mar 2022
DOIs	https://doi.org/10.1016/j.xpro.2022.101220
Publication status	Published - 18 Mar 2022

Keywords

Immunology
Metabolism
Microbiology
Model Organisms
Molecular Biology
Sequencing

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10.1016/j.xpro.2022.101220Licence: CC BY-NC-ND

Final published versionFinal published version, 2.71 MBLicence: CC BY-NC-ND

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title = "Protocol to assess the impact of early-life antibiotic exposure on murine longevity",

abstract = "We present this protocol using a mouse model to assess the impact of early-life antibiotic exposure on mammalian lifespan and the composition of the gut microbiota over time. We describe longitudinal fecal sampling and health monitoring following early-life antibiotic exposure. We detail DNA extraction and 16S rRNA gene sequencing to longitudinally profile the composition of the fecal microbiota. Finally, we discuss how to address potential confounders such as the stochastic recolonization of the gut microbiota following antibiotic exposure. For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021).",

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AU - Lynn, David J.

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AB - We present this protocol using a mouse model to assess the impact of early-life antibiotic exposure on mammalian lifespan and the composition of the gut microbiota over time. We describe longitudinal fecal sampling and health monitoring following early-life antibiotic exposure. We detail DNA extraction and 16S rRNA gene sequencing to longitudinally profile the composition of the fecal microbiota. Finally, we discuss how to address potential confounders such as the stochastic recolonization of the gut microbiota following antibiotic exposure. For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021).

KW - Immunology

KW - Metabolism

KW - Microbiology

KW - Model Organisms

KW - Molecular Biology

KW - Sequencing

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Protocol to assess the impact of early-life antibiotic exposure on murine longevity

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