Protocol to assess the impact of early-life antibiotic exposure on murine longevity

Miriam A. Lynn, Feargal J. Ryan, Yee C. Tee, Saoirse C. Benson, David J. Lynn

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Abstract

We present this protocol using a mouse model to assess the impact of early-life antibiotic exposure on mammalian lifespan and the composition of the gut microbiota over time. We describe longitudinal fecal sampling and health monitoring following early-life antibiotic exposure. We detail DNA extraction and 16S rRNA gene sequencing to longitudinally profile the composition of the fecal microbiota. Finally, we discuss how to address potential confounders such as the stochastic recolonization of the gut microbiota following antibiotic exposure. For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021).

Original languageEnglish
Article number101220
Number of pages22
JournalSTAR Protocols
Volume3
Issue number1
Early online date5 Mar 2022
DOIs
Publication statusPublished - 18 Mar 2022

Keywords

  • Immunology
  • Metabolism
  • Microbiology
  • Model Organisms
  • Molecular Biology
  • Sequencing

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