Pseudonocardia adelaidensis sp nov., an endophytic actinobacterium isolated from the surface-sterilized stem of a grey box tree (Eucalyptus microcarpa)

Onuma Kaewkla, Christopher Franco

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    An aerobic, actinobacterial strain with rod-shaped spores, EUM 221 T, which was isolated from the surface-sterilized stem of a grey box tree (Eucalyptus microcarpa), is described. Phylogenetic evaluation based on 16S rRNA gene sequence similarity showed that this isolate belongs to the family Pseudonocardiaceae, with the closest neighbour being Pseudonocardia zijingensis 6330T (98.7 %). The level of 16S rRNA gene sequence similarity between the isolate and species of the genus Pseudonocardia with validly published names ranged from 95 to 98 %. Chemotaxonomic data (meso-diaminopimelic acid; major menaquinone MK-8(H4); major fatty acid iso-C 16 : 0) confirmed the affiliation of strain EUM 221T to the genus Pseudonocardia. The results of the phylogenetic analysis, including physiological and biochemical studies in combination with DNA-DNA hybridization, allowed the genotypic and phenotypic differentiation of strain EUM 221 T from the closest described species. Therefore, this strain represented a novel species and the name proposed is Pseudonocardia adelaidensis sp. nov. The type strain is EUM 221T (=DSM 45352T =ACM 5286T).

    Original languageEnglish
    Pages (from-to)2818-2822
    Number of pages5
    JournalInternational Journal of Systematic and Evolutionary Microbiology
    Volume60
    Issue number12
    DOIs
    Publication statusPublished - 2010

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