Purification and characterization of a hydrogenase from the marine green alga Tetraselmis subcordiformis

Fei Yan, Zhaoan Chen, Wei Li, Xupeng Cao, Song Xue, Wei Zhang

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    7 Citations (Scopus)

    Abstract

    Soluble hydrogenase from a marine green alga, Tetraselmis subcordiformis, was purified to homogeneity and characterized under strict anaerobic conditions. After a 4 h dark, anaerobic adaptation period for the algal culture, the hydrogenase showed a maximum in vitro hydrogen production activity of 148 nmol H2/(μg Chl a × h). The enzyme was purified 362-fold by a three-step process, and using reduced methyl viologen as the electron donor its activity was 61.67 U/mg at 25 °C. The optimum temperature and pH value for hydrogen production were 55 °C and 7.5, respectively. The N-terminal amino acid sequence of this 46 kDa hydrogenase was determined, and it showed no homology to any known hydrogenase sequence.

    Original languageEnglish
    Pages (from-to)1212-1215
    Number of pages4
    JournalProcess Biochemistry
    Volume45
    Issue number5
    DOIs
    Publication statusPublished - 2011

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