Purification of trisomy 12 subclone in chronic lymphocytic leukaemia using imaging flow cytometry and fluorescence in situ hybridisation in suspension (FISH-IS): Poster ID: 340

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Abstract

Introduction: Trisomy of chromosome 12 is reported as one of the most common chromosomal aberrations in Chronic Lymphocytic Leukaemia (CLL), and confers an intermediate prognosis with a median overall survival of 114 months [1]. CLL cases with trisomy 12 are unique in their increased expression of integrins (such as CD49d and CD38) compared to other cytogenetic categories [2–4]. Trisomy 12 is also associated with mutations of NOTCH1 and a germline, unmutated configuration of the immunoglobulin heavy chain gene in NOTCH1 mutant cases [5,6].

These associations have however been inferred from analysis of whole populations of peripheral blood mononuclear cells (PBMCs) or CD19 positive B-lymphocytes. It is well established that a single patient’s CLL sample consists of a heterogeneous cell population, with multiple subclones present even at diagnosis, and that the subclonal composition and its evolution over time has a clinical impact [7–9]. We propose to use a novel methodology, combining fluorescence in situ hybridisation with flow cytometric sorting, to enable isolation and comprehensive analysis of the trisomic 12 CLL subclone.

Original languageEnglish
Article numberAbstract ID: 43
Pages (from-to)37-38
Number of pages2
JournalLeukemia and Lymphoma
Volume58
Issue numberS1
DOIs
Publication statusPublished - 15 Dec 2017
EventXVII International Workshop on Chronic Lymphocytic Leukemia - New York, United States
Duration: 12 May 201715 May 2017

Keywords

  • Conference abstract
  • Conference poster
  • Chronic Lymphocytic Leukaemia (CLL)
  • Trisomy 12
  • Trisomy of chromosome 12
  • increased expression of integrins
  • NOTCH1
  • mutations of NOTCH1

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