TY - JOUR
T1 - QTL mapping
T2 - an innovative method for investigating the genetic determinism of yeast-bacteria interactions in wine
AU - Bartle, Louise
AU - Peltier, Emilien
AU - Sundstrom, Joanna F.
AU - Sumby, Krista
AU - Mitchell, James G.
AU - Jiranek, Vladimir
AU - Marullo, Philippe
PY - 2021/6
Y1 - 2021/6
N2 - Abstract: The two most commonly used wine microorganisms, Saccharomyces cerevisiae yeast and Oenococcus oeni bacteria, are responsible for completion of alcoholic and malolactic fermentation (MLF), respectively. For successful co-inoculation, S. cerevisiae and O. oeni must be able to complete fermentation; however, this relies on compatibility between yeast and bacterial strains. For the first time, quantitative trait loci (QTL) analysis was used to elucidate whether S. cerevisiae genetic makeup can play a role in the ability of O. oeni to complete MLF. Assessment of 67 progeny from a hybrid S. cerevisiae strain (SBxGN), co-inoculated with a single O. oeni strain, SB3, revealed a major QTL linked to MLF completion by O. oeni. This QTL encompassed a well-known translocation, XV-t-XVI, that results in increased SSU1 expression and is functionally linked with numerous phenotypes including lag phase duration and sulphite export and production. A reciprocal hemizygosity assay was performed to elucidate the effect of the gene SSU1 in the SBxGN background. Our results revealed a strong effect of SSU1 haploinsufficiency on O. oeni’s ability to complete malolactic fermentation during co-inoculation and pave the way for the implementation of QTL mapping projects for deciphering the genetic bases of microbial interactions.
AB - Abstract: The two most commonly used wine microorganisms, Saccharomyces cerevisiae yeast and Oenococcus oeni bacteria, are responsible for completion of alcoholic and malolactic fermentation (MLF), respectively. For successful co-inoculation, S. cerevisiae and O. oeni must be able to complete fermentation; however, this relies on compatibility between yeast and bacterial strains. For the first time, quantitative trait loci (QTL) analysis was used to elucidate whether S. cerevisiae genetic makeup can play a role in the ability of O. oeni to complete MLF. Assessment of 67 progeny from a hybrid S. cerevisiae strain (SBxGN), co-inoculated with a single O. oeni strain, SB3, revealed a major QTL linked to MLF completion by O. oeni. This QTL encompassed a well-known translocation, XV-t-XVI, that results in increased SSU1 expression and is functionally linked with numerous phenotypes including lag phase duration and sulphite export and production. A reciprocal hemizygosity assay was performed to elucidate the effect of the gene SSU1 in the SBxGN background. Our results revealed a strong effect of SSU1 haploinsufficiency on O. oeni’s ability to complete malolactic fermentation during co-inoculation and pave the way for the implementation of QTL mapping projects for deciphering the genetic bases of microbial interactions.
KW - Malolactic fermentation
KW - QTL
KW - SSU1
KW - Wine
KW - Yeast-bacteria interactions
UR - http://www.scopus.com/inward/record.url?scp=85107502789&partnerID=8YFLogxK
U2 - 10.1007/s00253-021-11376-x
DO - 10.1007/s00253-021-11376-x
M3 - Article
C2 - 34106310
AN - SCOPUS:85107502789
SN - 0175-7598
VL - 105
SP - 5053
EP - 5066
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 12
ER -