Quantitative considerations for suspension array assays.

Ashley Connolly, Ramkumar Palanisamy, Matt Trau

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    A novel format of a multiplex microbead assay that is capable of accurately and reproducibly measuring small changes in 108 copies of a nucleic acid is reported herein. The dynamic range of the assay was 32-fold greater than conventional multiplex microbead assays. The assay was modeled mathematically which enabled the accuracy of DNA quantification to be formally analysed using a logical theoretical framework. The assay was also evaluated on RNA extracts taken from stimulated macrophages, to demonstrate that changes in gene expression can be accurately monitored in a biological system. We believe the reproducibility and accuracy of the assay presented herein will be useful for high-throughput, multiplex sensitive detection of nucleic acids over a large dynamic range in a research or diagnostic setting. Crown

    Original languageEnglish
    Pages (from-to)17-22
    Number of pages6
    JournalJournal of Biotechnology
    Volume145
    Issue number1
    DOIs
    Publication statusPublished - 1 Jan 2010

    Keywords

    • DNA hybridisation
    • DNA quantification
    • Flow cytometry
    • Immobilised oligonucleotides
    • Microbeads
    • Suspension arrays

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