Quantitative considerations for suspension array assays.

Ashley Connolly; Ramkumar Palanisamy; Matt Trau

doi:10.1016/j.jbiotec.2009.10.004

Quantitative considerations for suspension array assays.

Ashley Connolly, Ramkumar Palanisamy, Matt Trau

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A novel format of a multiplex microbead assay that is capable of accurately and reproducibly measuring small changes in 10⁸ copies of a nucleic acid is reported herein. The dynamic range of the assay was 32-fold greater than conventional multiplex microbead assays. The assay was modeled mathematically which enabled the accuracy of DNA quantification to be formally analysed using a logical theoretical framework. The assay was also evaluated on RNA extracts taken from stimulated macrophages, to demonstrate that changes in gene expression can be accurately monitored in a biological system. We believe the reproducibility and accuracy of the assay presented herein will be useful for high-throughput, multiplex sensitive detection of nucleic acids over a large dynamic range in a research or diagnostic setting. Crown

Original language	English
Pages (from-to)	17-22
Number of pages	6
Journal	Journal of Biotechnology
Volume	145
Issue number	1
DOIs	https://doi.org/10.1016/j.jbiotec.2009.10.004
Publication status	Published - 1 Jan 2010

Keywords

DNA hybridisation
DNA quantification
Flow cytometry
Immobilised oligonucleotides
Microbeads
Suspension arrays

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10.1016/j.jbiotec.2009.10.004

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KW - DNA hybridisation

KW - DNA quantification

KW - Flow cytometry

KW - Immobilised oligonucleotides

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KW - Suspension arrays

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Quantitative considerations for suspension array assays.

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Keywords

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