Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis

Kim Hare; R Marsh; M Binks; K Grimwood; Susan Pizzutto; Amanda Leach; Anne Chang; H Smith-Vaughan

doi:10.1016/j.mimet.2012.12.013

Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis

Kim Hare, R Marsh, M Binks, K Grimwood, Susan Pizzutto, Amanda Leach, Anne Chang, H Smith-Vaughan

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Correlation was observed between quantitative PCR and semi-quantitative culture for definition of Haemophilus influenzae infection in bronchoalveolar lavage specimens from 81 children with bronchiectasis. However, qPCR data correlated less well with airway neutrophilia, and supports continued use of culture as the gold standard for defining H. influenzae lower airway infection.

Original language	English
Pages (from-to)	270-272
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	92
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2012.12.013
Publication status	Published - Mar 2013

Keywords

Airway cellularity
Bronchiectasis
Estimates of infection
Haemophilus haemolyticus
Lower airway infection
Nontypeable Haemophilus influenzae

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10.1016/j.mimet.2012.12.013

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Hare, K., Marsh, R., Binks, M., Grimwood, K., Pizzutto, S., Leach, A., Chang, A., & Smith-Vaughan, H. (2013). Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis. Journal of Microbiological Methods, 92(3), 270-272. https://doi.org/10.1016/j.mimet.2012.12.013

Hare, Kim ; Marsh, R ; Binks, M ; Grimwood, K ; Pizzutto, Susan ; Leach, Amanda ; Chang, Anne ; Smith-Vaughan, H. / Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis. In: Journal of Microbiological Methods. 2013 ; Vol. 92, No. 3. pp. 270-272.

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abstract = "Correlation was observed between quantitative PCR and semi-quantitative culture for definition of Haemophilus influenzae infection in bronchoalveolar lavage specimens from 81 children with bronchiectasis. However, qPCR data correlated less well with airway neutrophilia, and supports continued use of culture as the gold standard for defining H. influenzae lower airway infection.",

keywords = "Airway cellularity, Bronchiectasis, Estimates of infection, Haemophilus haemolyticus, Lower airway infection, Nontypeable Haemophilus influenzae",

author = "Kim Hare and R Marsh and M Binks and K Grimwood and Susan Pizzutto and Amanda Leach and Anne Chang and H Smith-Vaughan",

year = "2013",

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Hare, K, Marsh, R, Binks, M, Grimwood, K, Pizzutto, S, Leach, A, Chang, A & Smith-Vaughan, H 2013, 'Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis', Journal of Microbiological Methods, vol. 92, no. 3, pp. 270-272. https://doi.org/10.1016/j.mimet.2012.12.013

Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis. / Hare, Kim; Marsh, R; Binks, M; Grimwood, K; Pizzutto, Susan; Leach, Amanda; Chang, Anne; Smith-Vaughan, H.

In: Journal of Microbiological Methods, Vol. 92, No. 3, 03.2013, p. 270-272.

Research output: Contribution to journal › Article › peer-review

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T1 - Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis

AU - Hare, Kim

AU - Marsh, R

AU - Binks, M

AU - Grimwood, K

AU - Pizzutto, Susan

AU - Leach, Amanda

AU - Chang, Anne

AU - Smith-Vaughan, H

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AB - Correlation was observed between quantitative PCR and semi-quantitative culture for definition of Haemophilus influenzae infection in bronchoalveolar lavage specimens from 81 children with bronchiectasis. However, qPCR data correlated less well with airway neutrophilia, and supports continued use of culture as the gold standard for defining H. influenzae lower airway infection.

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KW - Bronchiectasis

KW - Estimates of infection

KW - Haemophilus haemolyticus

KW - Lower airway infection

KW - Nontypeable Haemophilus influenzae

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JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

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ER -

Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis

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Keywords

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