Abstract
Correlation was observed between quantitative PCR and semi-quantitative culture for definition of Haemophilus influenzae infection in bronchoalveolar lavage specimens from 81 children with bronchiectasis. However, qPCR data correlated less well with airway neutrophilia, and supports continued use of culture as the gold standard for defining H. influenzae lower airway infection.
| Original language | English |
|---|---|
| Pages (from-to) | 270-272 |
| Number of pages | 3 |
| Journal | Journal of Microbiological Methods |
| Volume | 92 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2013 |
Keywords
- Airway cellularity
- Bronchiectasis
- Estimates of infection
- Haemophilus haemolyticus
- Lower airway infection
- Nontypeable Haemophilus influenzae
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Hare, K., Marsh, R., Binks, M., Grimwood, K., Pizzutto, S., Leach, A., Chang, A., & Smith-Vaughan, H. (2013). Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis. Journal of Microbiological Methods, 92(3), 270-272. https://doi.org/10.1016/j.mimet.2012.12.013