TY - JOUR
T1 - Race for the Surface
T2 - Eukaryotic Cells Can Win
AU - Pham, Vy T.H.
AU - Truong, Vi Khanh
AU - Orlowska, Anna
AU - Ghanaati, Shahram
AU - Barbeck, Mike
AU - Booms, Patrick
AU - Fulcher, Alex J.
AU - Bhadra, Chris M.
AU - Buividas, Ričardas
AU - Baulin, Vladimir
AU - James Kirkpatrick, C.
AU - Doran, Pauline
AU - Mainwaring, David E.
AU - Juodkazis, Saulius
AU - Crawford, Russell J.
AU - Ivanova, Elena P.
PY - 2016/8/31
Y1 - 2016/8/31
N2 - With an aging population and the consequent increasing use of medical implants, managing the possible infections arising from implant surgery remains a global challenge. Here, we demonstrate for the first time that a precise nanotopology provides an effective intervention in bacterial cocolonization enabling the proliferation of eukaryotic cells on a substratum surface, preinfected by both live Gram-negative, Pseudomonas aeruginosa, and Gram-positive, Staphylococcus aureus, pathogenic bacteria. The topology of the model black silicon (bSi) substratum not only favors the proliferation of eukaryotic cells but is biocompatible, not triggering an inflammatory response in the host. The attachment behavior and development of filopodia when COS-7 fibroblast cells are placed in contact with the bSi surface are demonstrated in the dynamic study, which is based on the use of real-time sequential confocal imaging. Bactericidal nanotopology may enhance the prospect for further development of inherently responsive antibacterial nanomaterials for bionic applications such as prosthetics and implants.
AB - With an aging population and the consequent increasing use of medical implants, managing the possible infections arising from implant surgery remains a global challenge. Here, we demonstrate for the first time that a precise nanotopology provides an effective intervention in bacterial cocolonization enabling the proliferation of eukaryotic cells on a substratum surface, preinfected by both live Gram-negative, Pseudomonas aeruginosa, and Gram-positive, Staphylococcus aureus, pathogenic bacteria. The topology of the model black silicon (bSi) substratum not only favors the proliferation of eukaryotic cells but is biocompatible, not triggering an inflammatory response in the host. The attachment behavior and development of filopodia when COS-7 fibroblast cells are placed in contact with the bSi surface are demonstrated in the dynamic study, which is based on the use of real-time sequential confocal imaging. Bactericidal nanotopology may enhance the prospect for further development of inherently responsive antibacterial nanomaterials for bionic applications such as prosthetics and implants.
KW - bactericidal surfaces
KW - competitive colonization
KW - inflammatory response
KW - microbial contamination
KW - nanostructures
UR - http://www.scopus.com/inward/record.url?scp=84985947117&partnerID=8YFLogxK
U2 - 10.1021/acsami.6b06415
DO - 10.1021/acsami.6b06415
M3 - Article
C2 - 27494044
AN - SCOPUS:84985947117
SN - 1944-8244
VL - 8
SP - 22025
EP - 22031
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 34
ER -