Abstract
A simple and rapid in vitro techinique to assess the sensitivity of human lymphocytes to X- and UV-radiation is decribes. Periphaela blood lymphocytes are cloned in agar and colony survival us used as the end point. Survival down to a fraction of apporox. 0.02 can be measures accurately. The D10 for X- radiation is apporox. 165 rad and the initial portion of the survival curve is linear with no evidence of a threshold. The D10 for UV-radiation is approx. 50 erg/mm2. The technique should be of value both for general radiobiological studies and fro detecting individuals having abnormalities of DNA repair.
Original language | English |
---|---|
Pages (from-to) | 125-131 |
Number of pages | 7 |
Journal | Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis |
Volume | 94 |
Issue number | 1 |
DOIs | |
Publication status | Published - May 1982 |
Externally published | Yes |