A simple and rapid in vitro techinique to assess the sensitivity of human lymphocytes to X- and UV-radiation is decribes. Periphaela blood lymphocytes are cloned in agar and colony survival us used as the end point. Survival down to a fraction of apporox. 0.02 can be measures accurately. The D10 for X- radiation is apporox. 165 rad and the initial portion of the survival curve is linear with no evidence of a threshold. The D10 for UV-radiation is approx. 50 erg/mm2. The technique should be of value both for general radiobiological studies and fro detecting individuals having abnormalities of DNA repair.
|Number of pages||7|
|Journal||Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis|
|Publication status||Published - May 1982|