Random non-coding fragments of lizard DNA: Anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species

Talat Mina Hojat Ansari Komachali; Terry Bertozzi; Jessica Hacking; Steven Cooper; Michael Gardner

doi:10.1071/ZO14085

Random non-coding fragments of lizard DNA: Anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species

Talat Mina Hojat Ansari Komachali, Terry Bertozzi, Jessica Hacking, Steven Cooper, Michael Gardner

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We report the development of 48 anonymous nuclear loci from the Australian skink Tiliqua rugosa using 454 sequencing. These loci amplified across a Western Australian lineage (47 loci), a 'northern' lineage (48 loci) and a 'southern' lineage (46 loci). We further tested amplification for the related T. adelaidensis and Egernia stokesii where 37 and 34 loci amplified respectively. The loci showed variability within T. rugosa (22 polymorphic loci) and at least 27 loci also exhibited variation among the three species, highlighting the usefulness of these markers for phylogenetic, phylogeographic and population genetic analyses in T. rugosa and related species.

Original language	English
Pages (from-to)	515-518
Number of pages	4
Journal	Australian Journal of Zoology
Volume	62
Issue number	6
DOIs	https://doi.org/10.1071/ZO14085
Publication status	Published - 2014

Keywords

DNA markers
phylogeography
squamate

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10.1071/ZO14085

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title = "Random non-coding fragments of lizard DNA: Anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species",

abstract = "We report the development of 48 anonymous nuclear loci from the Australian skink Tiliqua rugosa using 454 sequencing. These loci amplified across a Western Australian lineage (47 loci), a 'northern' lineage (48 loci) and a 'southern' lineage (46 loci). We further tested amplification for the related T. adelaidensis and Egernia stokesii where 37 and 34 loci amplified respectively. The loci showed variability within T. rugosa (22 polymorphic loci) and at least 27 loci also exhibited variation among the three species, highlighting the usefulness of these markers for phylogenetic, phylogeographic and population genetic analyses in T. rugosa and related species.",

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Random non-coding fragments of lizard DNA: Anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species. / Hojat Ansari Komachali, Talat Mina; Bertozzi, Terry; Hacking, Jessica et al.
In: Australian Journal of Zoology, Vol. 62, No. 6, 2014, p. 515-518.

Research output: Contribution to journal › Article › peer-review

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T2 - Anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species

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AU - Bertozzi, Terry

AU - Hacking, Jessica

AU - Cooper, Steven

AU - Gardner, Michael

PY - 2014

Y1 - 2014

N2 - We report the development of 48 anonymous nuclear loci from the Australian skink Tiliqua rugosa using 454 sequencing. These loci amplified across a Western Australian lineage (47 loci), a 'northern' lineage (48 loci) and a 'southern' lineage (46 loci). We further tested amplification for the related T. adelaidensis and Egernia stokesii where 37 and 34 loci amplified respectively. The loci showed variability within T. rugosa (22 polymorphic loci) and at least 27 loci also exhibited variation among the three species, highlighting the usefulness of these markers for phylogenetic, phylogeographic and population genetic analyses in T. rugosa and related species.

AB - We report the development of 48 anonymous nuclear loci from the Australian skink Tiliqua rugosa using 454 sequencing. These loci amplified across a Western Australian lineage (47 loci), a 'northern' lineage (48 loci) and a 'southern' lineage (46 loci). We further tested amplification for the related T. adelaidensis and Egernia stokesii where 37 and 34 loci amplified respectively. The loci showed variability within T. rugosa (22 polymorphic loci) and at least 27 loci also exhibited variation among the three species, highlighting the usefulness of these markers for phylogenetic, phylogeographic and population genetic analyses in T. rugosa and related species.

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JO - Australian Journal of Zoology

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IS - 6

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Random non-coding fragments of lizard DNA: Anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species

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Keywords

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