Random non-coding fragments of lizard DNA: anonymous nuclear markers for the Australian skink Sleepy Tiliqua rugosa , and their utility in other Egernia-group species: Anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species

Talat Mina Hojat Ansari Komachali, Terry Bertozzi, Jessica Hacking, Steven Cooper, Michael Gardner

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    We report the development of 48 anonymous nuclear loci from the Australian skink Tiliqua rugosa using 454 sequencing. These loci amplified across a Western Australian lineage (47 loci), a 'northern' lineage (48 loci) and a 'southern' lineage (46 loci). We further tested amplification for the related T. adelaidensis and Egernia stokesii where 37 and 34 loci amplified respectively. The loci showed variability within T. rugosa (22 polymorphic loci) and at least 27 loci also exhibited variation among the three species, highlighting the usefulness of these markers for phylogenetic, phylogeographic and population genetic analyses in T. rugosa and related species.

    Original languageEnglish
    Pages (from-to)515-518
    Number of pages4
    JournalAustralian Journal of Zoology
    Volume62
    Issue number6
    DOIs
    Publication statusPublished - 2014

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