TY - JOUR
T1 - Rapid Detection of Anabolic and Narcotic Doping Agents in Saliva and Urine by Means of Nanostructured Silicon SALDI Mass Spectrometry
AU - Minhas, Rajpreet Singh
AU - Rudd, David
AU - Al Hmoud, Hashim Z.
AU - Guinan, Taryn M.
AU - Kirkbride, K. Paul
AU - Voelcker, Nicolas H.
PY - 2020/7/15
Y1 - 2020/7/15
N2 - Novel doping agents and doping strategies are continually entering the market, placing a burden on analytical methods to detect, adapt, and respond to subtle changes in the composition of biological samples. Therefore, there is a growing interest in rapid, adaptable, and ideally confirmatory analytical methods for the fight against doping. Nanostructured silicon (nano-Si)-based surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) can effectively address this need, allowing fast and sensitive detection of prohibited compounds used in sport doping. Here, we demonstrate the detection of growth hormone peptides, anabolic-androgenic steroids, and narcotics at low concentrations directly from biological matrices. Molecular confirmation was performed using the fragmentation data of the structures, obtained with the tandem mass spectrometry capabilities of the SALDI instrument. The obtained data were in excellent agreement with those obtained using leading triple quadrupole liquid chromatography-mass spectrometry instruments. Furthermore, nano-Si SALDI-MS has the capacity for high-throughput analysis of hundreds of biological samples, providing opportunities for real-time MS analysis at sporting events.
AB - Novel doping agents and doping strategies are continually entering the market, placing a burden on analytical methods to detect, adapt, and respond to subtle changes in the composition of biological samples. Therefore, there is a growing interest in rapid, adaptable, and ideally confirmatory analytical methods for the fight against doping. Nanostructured silicon (nano-Si)-based surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) can effectively address this need, allowing fast and sensitive detection of prohibited compounds used in sport doping. Here, we demonstrate the detection of growth hormone peptides, anabolic-androgenic steroids, and narcotics at low concentrations directly from biological matrices. Molecular confirmation was performed using the fragmentation data of the structures, obtained with the tandem mass spectrometry capabilities of the SALDI instrument. The obtained data were in excellent agreement with those obtained using leading triple quadrupole liquid chromatography-mass spectrometry instruments. Furthermore, nano-Si SALDI-MS has the capacity for high-throughput analysis of hundreds of biological samples, providing opportunities for real-time MS analysis at sporting events.
KW - DIOS
KW - NIMS
KW - SALDI-MS
KW - detection
KW - doping
KW - mass spectrometry
KW - rapid
UR - http://www.scopus.com/inward/record.url?scp=85088276701&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/ARC/LP150100984
U2 - 10.1021/acsami.0c07849
DO - 10.1021/acsami.0c07849
M3 - Article
SN - 1944-8244
VL - 12
SP - 31195
EP - 31204
JO - ACS Applied Materials & Interfaces
JF - ACS Applied Materials & Interfaces
IS - 28
ER -