Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis

Steven Tong, Rachael Lilliebridge, Deborah Holt, Geoffrey Coombs, Bart Currie, Philip Giffard

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform.

    Original languageEnglish
    Pages (from-to)399-401
    Number of pages3
    JournalDiagnostic Microbiology and Infectious Disease
    Volume67
    Issue number4
    DOIs
    Publication statusPublished - Aug 2010

    Keywords

    • Australia
    • Community-associated methicillin-resistant Staphylococcus aureus
    • High-resolution melting
    • Panton-Valentine leukocidin
    • Staphylococcus aureus

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