We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform.
|Number of pages||3|
|Journal||Diagnostic Microbiology and Infectious Disease|
|Publication status||Published - Aug 2010|
- Community-associated methicillin-resistant Staphylococcus aureus
- High-resolution melting
- Panton-Valentine leukocidin
- Staphylococcus aureus