TY - JOUR
T1 - Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis
AU - Tong, Steven
AU - Lilliebridge, Rachael
AU - Holt, Deborah
AU - Coombs, Geoffrey
AU - Currie, Bart
AU - Giffard, Philip
PY - 2010/8
Y1 - 2010/8
N2 - We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform.
AB - We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform.
KW - Australia
KW - Community-associated methicillin-resistant Staphylococcus aureus
KW - High-resolution melting
KW - Panton-Valentine leukocidin
KW - Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=77954735663&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2010.03.015
DO - 10.1016/j.diagmicrobio.2010.03.015
M3 - Article
SN - 0732-8893
VL - 67
SP - 399
EP - 401
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 4
ER -