Rapid identification of the ABO genotypes by their single-stand conformation polymorphism

Li-Chin Tsai, Li-Gi Kao, Jan-Gowth Chang, Hsien-Hsiung Lee, Adrian Linacre, James Chun-I Lee

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The ABO locus on chromosome 9 contains many more alleles than are currently used routinely in forensic science. The use of singe-strand conformation polymorphism (SSCP) can separate sequence polymorphisms that differ by only one base. The SSCP process used allows for both single- and double-stranded polymerase chain reaction (PCR) products to be visualized. The six ABO genotypes can be differentiated by the allele-specific B and O SSCP patterns. The double-stranded DNA produced 'hybrid' bands due to heterozygous samples and allowed sequence diversity to be detected between alleles of heterozygotes. These 'hybrid' bands are valid markers to confirm genotypes of specimens.

Original languageEnglish
Pages (from-to)537-540
Number of pages4
JournalElectrophoresis
Volume21
Issue number3
DOIs
Publication statusPublished - 1 Feb 2000
Externally publishedYes

Keywords

  • ABO genotyping
  • Forensic DNA typing
  • Polymerase chain reaction
  • Single- strand conformation polymorphism

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