Rapid identification of the ABO genotypes by their single-stand conformation polymorphism

Li-Chin Tsai; Li-Gi Kao; Jan-Gowth Chang; Hsien-Hsiung Lee; Adrian Linacre; James Chun-I Lee

doi:10.1002/(SICI)1522-2683(20000201)21:3<537::AID-ELPS537>3.0.CO;2-1

Rapid identification of the ABO genotypes by their single-stand conformation polymorphism

Li-Chin Tsai, Li-Gi Kao, Jan-Gowth Chang, Hsien-Hsiung Lee, Adrian Linacre, James Chun-I Lee

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The ABO locus on chromosome 9 contains many more alleles than are currently used routinely in forensic science. The use of singe-strand conformation polymorphism (SSCP) can separate sequence polymorphisms that differ by only one base. The SSCP process used allows for both single- and double-stranded polymerase chain reaction (PCR) products to be visualized. The six ABO genotypes can be differentiated by the allele-specific B and O SSCP patterns. The double-stranded DNA produced 'hybrid' bands due to heterozygous samples and allowed sequence diversity to be detected between alleles of heterozygotes. These 'hybrid' bands are valid markers to confirm genotypes of specimens.

Original language	English
Pages (from-to)	537-540
Number of pages	4
Journal	Electrophoresis
Volume	21
Issue number	3
DOIs	https://doi.org/10.1002/(SICI)1522-2683(20000201)21:3<537::AID-ELPS537>3.0.CO;2-1
Publication status	Published - 1 Feb 2000
Externally published	Yes

Keywords

ABO genotyping
Forensic DNA typing
Polymerase chain reaction
Single- strand conformation polymorphism

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10.1002/(SICI)1522-2683(20000201)21:3<537::AID-ELPS537>3.0.CO;2-1

Cite this

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title = "Rapid identification of the ABO genotypes by their single-stand conformation polymorphism",

abstract = "The ABO locus on chromosome 9 contains many more alleles than are currently used routinely in forensic science. The use of singe-strand conformation polymorphism (SSCP) can separate sequence polymorphisms that differ by only one base. The SSCP process used allows for both single- and double-stranded polymerase chain reaction (PCR) products to be visualized. The six ABO genotypes can be differentiated by the allele-specific B and O SSCP patterns. The double-stranded DNA produced 'hybrid' bands due to heterozygous samples and allowed sequence diversity to be detected between alleles of heterozygotes. These 'hybrid' bands are valid markers to confirm genotypes of specimens.",

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AU - Tsai, Li-Chin

AU - Kao, Li-Gi

AU - Chang, Jan-Gowth

AU - Lee, Hsien-Hsiung

AU - Linacre, Adrian

AU - Lee, James Chun-I

PY - 2000/2/1

Y1 - 2000/2/1

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AB - The ABO locus on chromosome 9 contains many more alleles than are currently used routinely in forensic science. The use of singe-strand conformation polymorphism (SSCP) can separate sequence polymorphisms that differ by only one base. The SSCP process used allows for both single- and double-stranded polymerase chain reaction (PCR) products to be visualized. The six ABO genotypes can be differentiated by the allele-specific B and O SSCP patterns. The double-stranded DNA produced 'hybrid' bands due to heterozygous samples and allowed sequence diversity to be detected between alleles of heterozygotes. These 'hybrid' bands are valid markers to confirm genotypes of specimens.

KW - ABO genotyping

KW - Forensic DNA typing

KW - Polymerase chain reaction

KW - Single- strand conformation polymorphism

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Rapid identification of the ABO genotypes by their single-stand conformation polymorphism

Abstract

Keywords

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