Rapid protein immobilization for thin film continuous flow biocatalysis

Joshua Britton, Colin Raston, Gregory Weiss

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    A versatile enzyme immobilization strategy for thin film continuous flow processing is reported. Here, non-covalent and glutaraldehyde bioconjugation are used to immobilize enzymes on the surfaces of borosilicate reactors. This approach requires only ng of protein per reactor tube, with the stock protein solution readily recycled to sequentially coat >10 reactors. Confining reagents to thin films during immobilization reduced the amount of protein, piranha-cleaning solution, and other reagents by ∼96%. Through this technique, there was no loss of catalytic activity over 10 h processing. The results reported here combines the benefits of thin film flow processing with the mild conditions of biocatalysis.

    Original languageEnglish
    Pages (from-to)10159-10162
    Number of pages4
    JournalChemical Communications
    Volume52
    Issue number52
    DOIs
    Publication statusPublished - 2016

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