Rat cardiac neurons express the non-coding R-exon (exon 1) of the cholinergic gene locus

Wolfgang Kummer; Ludger Fink; Magdalena Dvorakova; Rainer Haberberger; Rainer M. Bohle

doi:10.1097/00001756-199807130-00011

Rat cardiac neurons express the non-coding R-exon (exon 1) of the cholinergic gene locus

Wolfgang Kummer, Ludger Fink, Magdalena Dvorakova, Rainer Haberberger, Rainer M. Bohle

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Choline acetyltransferase (ChAT) and the vesicular acetylcholine transporter (VAChT) are both encoded by the cholinergic gene locus from which, in the rat, five different species of ChAT mRNA and three different species of VAChT mRNA are produced. So far, discrimination between mRNA subtypes has been possible only in CNS homogenates or in cell cultures. In this study, cardiac neurons were microdissected from frozen sections of rat heart using a u.v. laser and harvested using a micromanipulator. RT-PCR demonstrated the expression of the non-coding R-exon and splicing to R1-type mRNA in the majority of cardiac neurons. The technique presented here is the first to allow subtype analysis of cholinergic locus mRNA species in neurons in situ.

Original language	English
Pages (from-to)	2209-2212
Number of pages	4
Journal	Neuroreport
Volume	9
Issue number	10
DOIs	https://doi.org/10.1097/00001756-199807130-00011
Publication status	Published - 13 Jul 1998
Externally published	Yes

Keywords

Cardiac ganglia
Cell picking
Choline acetyltransferase
Cholinergic gene locus
Heart
mRNA
Polymerase chain reaction
Rat

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abstract = "Choline acetyltransferase (ChAT) and the vesicular acetylcholine transporter (VAChT) are both encoded by the cholinergic gene locus from which, in the rat, five different species of ChAT mRNA and three different species of VAChT mRNA are produced. So far, discrimination between mRNA subtypes has been possible only in CNS homogenates or in cell cultures. In this study, cardiac neurons were microdissected from frozen sections of rat heart using a u.v. laser and harvested using a micromanipulator. RT-PCR demonstrated the expression of the non-coding R-exon and splicing to R1-type mRNA in the majority of cardiac neurons. The technique presented here is the first to allow subtype analysis of cholinergic locus mRNA species in neurons in situ.",

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AU - Kummer, Wolfgang

AU - Fink, Ludger

AU - Dvorakova, Magdalena

AU - Haberberger, Rainer

AU - Bohle, Rainer M.

PY - 1998/7/13

Y1 - 1998/7/13

N2 - Choline acetyltransferase (ChAT) and the vesicular acetylcholine transporter (VAChT) are both encoded by the cholinergic gene locus from which, in the rat, five different species of ChAT mRNA and three different species of VAChT mRNA are produced. So far, discrimination between mRNA subtypes has been possible only in CNS homogenates or in cell cultures. In this study, cardiac neurons were microdissected from frozen sections of rat heart using a u.v. laser and harvested using a micromanipulator. RT-PCR demonstrated the expression of the non-coding R-exon and splicing to R1-type mRNA in the majority of cardiac neurons. The technique presented here is the first to allow subtype analysis of cholinergic locus mRNA species in neurons in situ.

AB - Choline acetyltransferase (ChAT) and the vesicular acetylcholine transporter (VAChT) are both encoded by the cholinergic gene locus from which, in the rat, five different species of ChAT mRNA and three different species of VAChT mRNA are produced. So far, discrimination between mRNA subtypes has been possible only in CNS homogenates or in cell cultures. In this study, cardiac neurons were microdissected from frozen sections of rat heart using a u.v. laser and harvested using a micromanipulator. RT-PCR demonstrated the expression of the non-coding R-exon and splicing to R1-type mRNA in the majority of cardiac neurons. The technique presented here is the first to allow subtype analysis of cholinergic locus mRNA species in neurons in situ.

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KW - Cell picking

KW - Choline acetyltransferase

KW - Cholinergic gene locus

KW - Heart

KW - mRNA

KW - Polymerase chain reaction

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Rat cardiac neurons express the non-coding R-exon (exon 1) of the cholinergic gene locus

Abstract

Keywords

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