Recent developments in nucleic acid identification using solid-phase enzymatic assays

Dmitriy Khodakov, Amanda Ellis

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    This review (containing 101 refs.) covers recent achievements in the development of new approaches for enzymatically assisted detection of nucleic acids on microarrays. We discuss molecular techniques including the polymerase chain reaction, reverse transcription, allele specific primer extension and a range of isothermal techniques for the amplification and discrimination of nucleic acids. This also includes their implementation into microfluidic systems. These techniques all show great promise for use in the life sciences by allowing for high throughput, cost effective and highly sensitive and specific analysis of nucleic acids. Importantly, they can be potentially integrated into personalized and point-of-care medicine.

    Original languageEnglish
    Pages (from-to)1633-1646
    Number of pages14
    JournalMICROCHIMICA ACTA
    Volume181
    Issue number13-14
    DOIs
    Publication statusPublished - 2014

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