Recent insights into microbial catalases: Isolation, production and purification

Balwinder Sooch, Baljinder Kauldhar, Munish Puri

    Research output: Contribution to journalReview articlepeer-review

    31 Citations (Scopus)

    Abstract

    Catalase, an oxidoreductase enzyme, works as a detoxification system inside living cells against reactive oxygen species formed as a by-product of different metabolic reactions. The enzyme is found in a wide range of aerobic and anaerobic organisms. Catalase has also been employed in various analytical and diagnostic methods in the form of biosensors and biomarkers in addition to its other applications in textile, paper, food and pharmaceutical industries. New applications for catalases are constantly emerging thanks to their high turnover rate, distinct evolutionary origin, relatively simple and well-defined reaction mechanisms. The following review provides comprehensive information on isolation, production and purification of catalases with different techniques from various microbial sources along with their types, structure, mechanism of action and applications.

    Original languageEnglish
    Pages (from-to)1429-1447
    Number of pages19
    JournalBiotechnology Advances
    Volume32
    Issue number8
    DOIs
    Publication statusPublished - 1 Dec 2014

    Keywords

    • Catalase peroxidase
    • Downstream processing
    • Haem-enzyme
    • Hydrogen peroxide
    • Oxido-reductase
    • Protein structure
    • Purification

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